Hu IL-6 Recom Protein 10ug

Interleukin-6 (IL-6) is a potent lymphoid cell growth factor which affects B lymphocytes, T lymphocytes, and hybridoma cells.  It will also affect cytotoxic T cells in combination with other factors, such as IL-2 and interferon-γ.  Human IL-6 is a 20.5 kD protein containing 184 amino acid residues.  Recombinant human IL-6 (Cat. No. 550071) is supplied as a frozen liquid comprised of 0.22 µm filtered aqueous buffered solution, glycerol and bovine serum albumin, with no preservatives.  Recombinant human IL-6 is ≥ 95% pure as determined by SDS-PAGE, and an absorbance assay based on the Beers-Lambert law.  The endotoxin level is  ≤0.1 ng per µg of human IL-6, as measured in a chromogenic LAL assay.

200 µg/ml

ELISA Standard (Routinely Tested), Bioassay, Blocking (Tested During Development)

Human (QC Testing)

IL-6

Frozen aqueous buffered solution containing BSA and glycerol.

Development References(4) 1. Hirano T, Yasukawa K, Harada H, et al.. Complementary DNA for a novel human interleukin (BSF-2) that induces B lymphocytes to produce immunoglobulin. Nature. 1986; 324(6092):73-76. (Biology). 2. Kitamura T, Takaku F, Miyajima A. IL-1 up-regulates the expression of cytokine receptors on a factor-dependent human hemopoietic cell line, TF-1. Int Immunol. 1991; 3(6):571-577. (Biology). 3. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Methodology). 4. Van Snick J. Interleukin-6: an overview. Annu Rev Immunol. 1990; 8:253-278. (Biology).