老带新
hIL-6 Aff Pur PAb (100 ug)
1/8

hIL-6 Aff Pur PAb (100 ug)

分享
品牌: R&D Systems
pdf 下载产品说明书
pdf 下载SDS
用小程序,查商品更便捷
收藏
对比对比
咨询咨询
来源宿主:
Goat IgG
实验应用:
Western Blot(0.2 µg/mL)
Immunocytochemistry(5-15 µg/mL)
产品介绍
产品介绍
产品信息
荧光素标记
纯化方式
Antigen Affinity-purified
抗原名称
B-cell differentiation factor, B-cell stimulatory factor 2, BSF2, BSF-2, CDF, CTL differentiation fa
宿主
Goat IgG
免疫原
E. coli-derived recombinant human IL-6
Pro29-Met212
Accession # P05231

Scientific Data

Immunocytochemistry IL-6 antibody in Human PBMCs by Immunocytochemistry (ICC).View Larger

IL‑6 in Human PBMCs. IL-6 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS and monensin using Goat Anti-Human IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-206-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Neutralization Cell Proliferation Induced by IL‑6 and Neutralization by Human IL‑6 Antibody.View Larger

Cell Proliferation Induced by IL‑6 and Neutralization by Human IL‑6 Antibody. Recombinant Human IL-6 (Catalog # 206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-206-NA). The ND50 is typically ≤ 125 ng/mL.

Immunohistochemistry Detection of Human IL-6 by ImmunohistochemistryView Larger

Detection of Human IL-6 by Immunohistochemistry Breast DCIS cells overexpress proinflammatory markers. Representative images of immunohistochemistry targeting IL-6 protein in normal breast tissue (a), and breast DCIS (b) (N = 61). c and d Hematoxylin staining of serial sections from tissue shown in panel A and B. All images are 20X magnification. Scale bar equals 100 μm. e Evaluation of IL-6 gene expression in DCIS cells via qRT-PCR; the isogenic MCF10.DCIS cells and the non-isogenic SUM102 cell line were analyzed against the non-transformed MCF10A cell line (N = 3). f Secretion of IL-6 protein from DCIS cell lines and non-transformed MCF10A cells as determined by ELISA. *P < 0.05, Student’s t-test; mean ± SD Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26268945), licensed under a CC-BY license. Not internally tested by R&D Systems.

Block/ Neutralize Detection of Human IL-6 by Block/NeutralizeView Larger

Detection of Human IL-6 by Block/Neutralize IL-6 neutralizing antibody (nAb) inhibits growth of MCF10.DCIS structures. Representative contiguous 16-tiled DIC images of MCF10.DCIS cells grown in MAME cultures for 8 days. MAME culture of MCF10.DCIS cells treated with an isotype-matched antibody against IgG (control) (8 days) (a), or 1 μg /ml IL-6 nAb (b) (8 days). MCF10.DCIS cells treated with IL-6 nAb followed by a 48 h treatment-free recovery period prior to imaging on day 10 (c). N = 3, Scale bars, 200 μm. d Diameter of MCF10.DCIS structures in the presence of control antibody, IL-6 nAb, or 48 h recovery from IL-6 nAb. N = 20-40 measurements /tiled DIC image (N = 3). ****P ≤ 0.0001, **P ≤ 0.01. e Evaluation of gene expression of invasive tumor cell markers in MAME cultures treated with IL-6 nAb (N = 3). Fold difference as compared to control cultures. Dashed line indicates 2-fold threshold. Student’s t-test; mean ± SD Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26268945), licensed under a CC-BY license. Not internally tested by R&D Systems.

Block/ Neutralize Detection of Human IL-6 by Block/NeutralizeView Larger

Detection of Human IL-6 by Block/Neutralize IL-6 nAb inhibits CAF-stimulated MCF10.DCIS structure growth and results in altered morphology. Contiguous 16-tile DIC image of CAFs: WS12Ti (A, B) or CAF40TKi (c, d) co-cultured with MCF10.DCIS cells for 8 days in the presence of isotype matched anti-IgG (a, c) or 1 μg /ml IL-6 nAb (b, d). e Perimeter measurements from DCIS structures in MAME culture in the presence of anti-IgG or IL-6 nAb (N = 100-140 measurements from 6 contiguous tiled DIC images (N = 3). Fluorescent tiled images of CAF40TKi fibroblasts (unlabeled) cultured with MCF10.DCIS-RFP cells in the presence of anti-IgG (f) or 1 μg /ml IL-6 nAb (g). h Quantification of total volume of DCIS-RFP structures in co-culture with CAF40TKi fibroblasts (ratio-paired t-test, N = 3). ***P ≤ 0.001, **P ≤ 0.01; Student’s t-test; mean ± SD Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26268945), licensed under a CC-BY license. Not internally tested by R&D Systems.

Block/ Neutralize Detection of Human IL-6 by Block/NeutralizeView Larger

Detection of Human IL-6 by Block/Neutralize IL-6 neutralizing antibody (nAb) inhibits growth of MCF10.DCIS structures. Representative contiguous 16-tiled DIC images of MCF10.DCIS cells grown in MAME cultures for 8 days. MAME culture of MCF10.DCIS cells treated with an isotype-matched antibody against IgG (control) (8 days) (a), or 1 μg /ml IL-6 nAb (b) (8 days). MCF10.DCIS cells treated with IL-6 nAb followed by a 48 h treatment-free recovery period prior to imaging on day 10 (c). N = 3, Scale bars, 200 μm. d Diameter of MCF10.DCIS structures in the presence of control antibody, IL-6 nAb, or 48 h recovery from IL-6 nAb. N = 20-40 measurements /tiled DIC image (N = 3). ****P ≤ 0.0001, **P ≤ 0.01. e Evaluation of gene expression of invasive tumor cell markers in MAME cultures treated with IL-6 nAb (N = 3). Fold difference as compared to control cultures. Dashed line indicates 2-fold threshold. Student’s t-test; mean ± SD Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26268945), licensed under a CC-BY license. Not internally tested by R&D Systems.

Block/ Neutralize Detection of Human IL-6 by Block/NeutralizeView Larger

Detection of Human IL-6 by Block/Neutralize IL-6 nAb inhibits CAF-stimulated MCF10.DCIS structure growth and results in altered morphology. Contiguous 16-tile DIC image of CAFs: WS12Ti (A, B) or CAF40TKi (c, d) co-cultured with MCF10.DCIS cells for 8 days in the presence of isotype matched anti-IgG (a, c) or 1 μg /ml IL-6 nAb (b, d). e Perimeter measurements from DCIS structures in MAME culture in the presence of anti-IgG or IL-6 nAb (N = 100-140 measurements from 6 contiguous tiled DIC images (N = 3). Fluorescent tiled images of CAF40TKi fibroblasts (unlabeled) cultured with MCF10.DCIS-RFP cells in the presence of anti-IgG (f) or 1 μg /ml IL-6 nAb (g). h Quantification of total volume of DCIS-RFP structures in co-culture with CAF40TKi fibroblasts (ratio-paired t-test, N = 3). ***P ≤ 0.001, **P ≤ 0.01; Student’s t-test; mean ± SD Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26268945), licensed under a CC-BY license. Not internally tested by R&D Systems.

Immunohistochemistry Detection of Human IL-6 by ImmunohistochemistryView Larger

Detection of Human IL-6 by Immunohistochemistry Breast DCIS cells overexpress proinflammatory markers. Representative images of immunohistochemistry targeting IL-6 protein in normal breast tissue (a), and breast DCIS (b) (N = 61). c and d Hematoxylin staining of serial sections from tissue shown in panel A and B. All images are 20X magnification. Scale bar equals 100 μm. e Evaluation of IL-6 gene expression in DCIS cells via qRT-PCR; the isogenic MCF10.DCIS cells and the non-isogenic SUM102 cell line were analyzed against the non-transformed MCF10A cell line (N = 3). f Secretion of IL-6 protein from DCIS cell lines and non-transformed MCF10A cells as determined by ELISA. *P < 0.05, Student’s t-test; mean ± SD Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26268945), licensed under a CC-BY license. Not internally tested by R&D Systems.

Human IL-6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human IL-6 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse IL-6 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human IL-6
Pro29-Met212
Accession # P05231
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. Nordan, R.P. and M. Potter (1986) Science 233:566. The Neutralization Dose (ND50) is typically ≤ 125 ng/mL in the presence of 2.5 ng/mL Recombinant Human IL‑6.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

应用
实验应用
Western Blot(0.2 µg/mL)
Immunocytochemistry(5-15 µg/mL)
背景
别名
B-cell differentiation factor,B-cell stimulatory factor 2,BSF2,BSF-2,CDF,CTL differentiation factor ,HSF,hybridoma growth factor,IFNB2,IFN-beta-2,IL6,IL-6,Interferon beta-2,interleukin 6 (interferon, beta 2),interleukin BSF-2,interleukin-6,MGI-2A,Interleukin 6
背景

Background: IL-6

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. Mature human IL-6 is 183 amino acids (aa) in length expressed as a 22-28 kDA molecular weight protein. IL-6 shares 39% aa sequence identity with mouse and rat IL-6. Alternative splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties. IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor. IL-6, along with TNF-alpha and IL-1, function to drive the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis. IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise. In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells.

Long Name
Interleukin 6
Entrez Gene IDs
3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 102138971 (Cynomolgus Monkey); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names
B-cell differentiation factor; B-cell stimulatory factor 2; BSF2; BSF-2; CDF; CTL differentiation factor ; HSF; hybridoma growth factor; IFNB2; IFN-beta-2; IL6; IL-6; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A
制备和贮存
溶解方法
Reconstitute at 0.2 mg/mL in sterile PBS.
保存方式

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

参考图片

Detection of Human IL‑6 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) untreated (-) or treated (+) with PHA. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-206-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL‑6 at approximately 20-22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

IL‑6 in Human PBMCs. IL‑6 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS and monensin using Goat Anti-Human IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-206-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter­stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Cell Proliferation Induced by IL‑6 and Neutralization by Human IL‑6 Antibody. Recombinant Human IL‑6 (Catalog # 206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL‑6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-206-NA). The ND50 is typically 15-45 ng/mL.

声明 :本官网所有报价均为常温或者蓝冰运输价格,如有产品需要干冰运输,需另外加收干冰运输费。
货号:
AF-206-NA
一键复制
询价
25ug
100ug
选择数量
当前规格1件起购 
配送至
预计2-3周送达,快递: 免运费,若需干冰额外收费