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Product Summary
The Quantikine HS Human IL-7 Immunoassay is a solid phase ELISA that specifically measures IL-7 in 18-24 hours (with an overnight incubation step). This kit is designed to measure IL-7 levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human IL-7 and antibodies raised against the recombinant factor. It has been shown to quantitate the recombinant factor accurately. Results obtained using natural IL-7 showed linear curves that were parallel to the standard curves obtained using the kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-7.
Recovery
The recovery of IL-7 spiked to three different levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 103 | 89-113 |
| Citrate Plasma (n=5) | 100 | 92-110 |
| EDTA Plasma (n=5) | 112 | 83-132 |
| Serum (n=5) | 100 | 91-113 |
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of IL-7 and diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data
Assay Procedure
Refer to the product Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 200 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 14-20 hours.
- Aspirate each well and wash, repeating the process 5 times for a total of 6 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 6 times.
- Add 50 µL Substrate Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 45 minutes. Do not wash the plate.
- Add 50 µL Amplifier Solution to each well. Cover with a new plate sealer, and incubate at room temperature for 45 minutes.
- Add 50 µL of Stop Solution to each well. Read at 490 nm within 30 minutes. Set wavelength correction to 650 nm or 690 nm.
50 µL Assay Diluent
200 µL Standard, Control, or Sample
200 µL Conjugate
50 µL Substrate Solution
50 µL Amplifier Solution
50 µL Stop Solution
Human IL-7 Quantikine HS ELISA Kit Summary
Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
18-24 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (200 uL), Serum (200 uL), EDTA Plasma (200 uL), Citrate Plasma (200 uL)
Sensitivity
0.1 pg/mL
Assay Range
0.2 - 10 pg/mL (Cell Culture Supernates), 0.3 - 16 pg/mL (Serum, EDTA Plasma, Citrate Plasma)
Specificity
Natural and recombinant human IL-7
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
背景
别名
IL7,IL-7,IL-7interleukin-7,interleukin 7,Lymphopoietin-1,PBGF,Interleukin 7
背景
Background: IL-7
IL-7 (Interleukin-7) is a cytokine that plays important roles in lymphocyte differentiation, proliferation, and survival. IL-7 is produced by stromal epithelial cells of the thymus, bone marrow, and intestines. It signals through a receptor complex composed of IL-7 R alpha/CD127 and the Common gamma Chain. gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. IL-7 contributes to the maintenance of all naïve and memory T cells. It is required for optimal T cell-dendritic cell interaction. In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development.
Long Name:
Interleukin 7
Entrez Gene IDs:
3574 (Human); 16196 (Mouse); 25647 (Rat)
Alternate Names:
IL7; IL-7; IL-7interleukin-7; interleukin 7; Lymphopoietin-1; PBGF
研究领域
制备和贮存
保存方式
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
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