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Human IL-7 Quantikine HS ELISA Kit (1 Kit)
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Human IL-7 Quantikine HS ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 0.5 | 1.49 | 6.86 | 0.78 | 2.13 | 8.81 |
| Standard Deviation | 0.04 | 0.14 | 0.61 | 0.08 | 0.16 | 0.64 |
| CV% | 8 | 9.4 | 8.9 | 10.3 | 7.5 | 7.3 |
Serum, EDTA Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 0.46 | 5.79 | 11.18 | 0.51 | 6.38 | 12.95 |
| Standard Deviation | 0.03 | 0.19 | 0.44 | 0.06 | 0.5 | 1.3 |
| CV% | 6.5 | 3.3 | 3.9 | 11.8 | 7.8 | 10 |
Recovery
The recovery of IL-7 spiked to three different levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=5) | 103 | 89-113 |
| Citrate Plasma (n=5) | 100 | 92-110 |
| EDTA Plasma (n=5) | 112 | 83-132 |
| Serum (n=5) | 100 | 91-113 |
Linearity
Scientific Data
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Human IL-7 High Sensitivity ELISA Cell Culture Supernate Standard Curve
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Human IL-7 High Sensitivity ELISA Serum/Plasma Standard Curve
Product Datasheets
Preparation and Storage
Background: IL-7
IL-7 (Interleukin-7) is a cytokine that plays important roles in lymphocyte differentiation, proliferation, and survival. IL-7 is produced by stromal epithelial cells of the thymus, bone marrow, and intestines. It signals through a receptor complex composed of IL-7 R alpha/CD127 and the Common gamma Chain. gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. IL-7 contributes to the maintenance of all naïve and memory T cells. It is required for optimal T cell-dendritic cell interaction. In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development.
Background: IL-7
IL-7 (Interleukin-7) is a cytokine that plays important roles in lymphocyte differentiation, proliferation, and survival. IL-7 is produced by stromal epithelial cells of the thymus, bone marrow, and intestines. It signals through a receptor complex composed of IL-7 R alpha/CD127 and the Common gamma Chain. gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. IL-7 contributes to the maintenance of all naïve and memory T cells. It is required for optimal T cell-dendritic cell interaction. In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development.