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rhIL-8, CF (10 ug)
rhIL-8, CF (10 ug)
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rhIL-8, CF (10 ug)

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    Scientific Data

    Bioactivity Recombinant Human IL-8/CXCL8 Protein BioactivityView Larger

    Recombinant Human IL-8/CXCL8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2. The ED50 for this effect is 0.5-2.5 ng/mL.

    SDS-PAGE Recombinant Human IL-8/CXCL8 Protein SDS-PAGEView Larger

    1 μg/lane of Recombinant Human IL-8/CXCL8 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 8 kDa.

    Carrier Free

    What does CF mean?

    CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

    What formulation is right for me?

    In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

    208-IL

    FormulationLyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
    ReconstitutionReconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
    ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

    208-IL/CF

    FormulationLyophilized from a 0.2 μm filtered solution in PBS.
    ReconstitutionReconstitute at 100 μg/mL in sterile PBS.
    ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

    Recombinant Human IL-8/CXCL8 Protein Summary

    Product Specifications

    Purity
    >97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
    Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
    Activity
    Measured by its ability to chemoattract BaF3 mouse pro‑B cells transfected with human CXCR2. The ED50 for this effect is 0.5-2.5 ng/mL.
    Source
    E. coli-derived human IL-8/CXCL8 protein
    Ser28-Ser99
    Accession #
    P10145.1
    N-terminal Sequence
    Analysis
    Ser28
    Predicted Molecular Mass
    8 kDa
    背景
    背景

    Background: IL-8/CXCL8

    Interleukin-8 (IL-8), also known as CXCL8, GCP-1, and NAP-1, is a widely expressed proinflammatory member of the CXC family of chemokines. Near its N-terminus, this 8-9 kDa chemokine contains an ELR motif which is important for its angiogenic properties (1). IL-8/CXCL8 can associate into a homodimer or a heterodimer with CXCL4/PF4 (2), and it can also interact with matrix and cell surface glycosaminoglycans (3). Mature human IL-8/CXCL8 shares 65%-69% amino acid (aa) sequence identiity with canine, feline, and porcine IL-8/CXCL8 (4). There is no IL-8/CXCL8 gene counterpart in rodent. N-terminal truncation by multiple proteases generates a range of shorter forms, and an alternative splice form of human IL-8/CXCL8 carries an eleven aa substitution at the C-terminus (5). The bioactivity of IL-8/CXCL8 is regulated by these truncations, by IL-8/CXCL8 citrullination at Arg5 (N-terminal to the ELR motif) (6), and by the decoy receptor DARC (7). IL-8/CXCL8 effects are mediated through CXCR1/IL-8 RA, which is also used by CXCL6, and through CXCR2/IL-8 RB, which is used by multiple CXC chemokines (1). CXCR1 and CXCR2 associate into functional homodimers and heterodimers with each other (8). Through both CXCR1 and CXCR2, CXCL8 promotes neutrophil adhesion to the vascular endothelium and migration to sites of inflammation (9). It triggers the antimicrobial activation of neutrophils through CXCR1 (10). CXCL8 also binds to Serpin A1/alpha-1 Antitrypsin, and this prevents IL-8/CXCL8 interaction with CXCR1 (11). IL-8/CXCL8 is upregulated in atherosclerotic lesions and other cardiac pathologies where it exacerbates inflammatory tissue damage (12). In addition, it induces VEGF expression, vascular endothelial cell proliferation, angiogenesis, and tumor cell invasiveness (13-16).

    References
    1. Lazennec, G. and A. Richmond (2010) Trends Mol. Med. 16:133.
    2. Nesmelova, I.V. et al. (2005) J. Biol. Chem. 280:4948.
    3. Pichert, A. et al. (2012) Biomatter 2:142.
    4. Schmid, J. and C. Weissmann (1987) J. Immunol. 139:250.
    5. Mortier, A. et al. (2008) Pharmacol. Ther. 120:197.
    6. Proost, P. et al. (2008) J. Exp. Med. 205:2085.
    7. Neote, K. et al. (1994) Blood 84:44.
    8. Munoz, L.M. et al. (2009) J. Immunol. 183:7337.
    9. Gerszten, R.E. et al. (1999) Nature 398:718.
    10. Jones, S.A. et al. (1996) Proc. Natl. Acad. Sci. USA 93:6682.
    11. Bergin, D.A. et al. (2010) J. Clin. Invest. 120:4236.
    12. Apostolakis, S. et al. (2009) Cardiovasc. Res. 84:353.
    13. Martin, D. et al. (2009) J. Biol. Chem. 284:6038.
    14. Li, A. et al. (2005) Angiogenesis 8:63.
    15. Waugh, D.J. and C. Wilson (2008) Clin. Cancer Res. 14:6735.
    16. Fernando, R.I. et al. (2011) Cancer Res. 71:5296.
    Long Name
    Interleukin 8
    Entrez Gene IDs
    3576 (Human); 396880 (Porcine); 403850 (Canine); 493836 (Feline)
    Alternate Names
    3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; LUCT; LYNAP; MDNCF; MDNCFb-ENAP; member 8; MONAP; MONAPGCP1; NAF; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

    参考图片

    1 μg/lane of Recombinant Human CXCL8/IL‑8 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 8 kDa.

    Recombinant Human CXCL8/IL-8 (Catalog # 208-IL) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR2. The ED50 for this effect is typically 0.5-2.5 ng/mL.

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    货号：
    208-IL-010/CF
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