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In situ Hybridization Kit BioAssay™ (ISH)
In situ Hybridization Kit BioAssay™ (ISH)
品牌： Usbiological
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咨询实验应用：
• Gene mutation studies • Protein expression and function analysis
产品介绍
产品介绍
产品信息
产品用途
• Gene mutation studies • Protein expression and function analysis
简单描述
In situ hybridization (ISH) is a powerful method for gene expression analysis. This In situ Hybridization Kit (ISH), BioAssay™ provides the reagents, including hybridization buffer, NBT/BCIP color development solution and washing buffer solutions, for pre-hybridization, hybridization, and post hybridization treatments. This ISH kit provides researchers a method doing in situ hybridization with non-radioactive probes. Compared with radioactive methods, the sensitivity of this kit is the same while the procedure is simplified. This kit is suitable for digoxigenin labled probes combined with an alkaline phosphatase color development system. Using this kit, all disadvantages of working with radioactive materials can be avoided.
组成成分
I7643-50A: Prehybridization Solution, 1x2ml or 1x5ml I7643-50B: Hybridization Solution, 1x2ml or 1x5ml I7643-50C: NBT, 1x120ul or 1x300ul I7643-50D: BCIP, 1x60ul or 1x150ul I7643-50E: SSC Buffer, 20X, 1x20ml or 1x50ml I7643-50F: Alkaline Phosphatase Buffer, 10X, 1x20ml or 1x50ml I7643-50G: anti-Dig Antibody, 1x10ul or 1x25ul I7643-50H: Blocking Solution, 100X, 1x100ul or 1x250ul
应用
实验应用
• Gene mutation studies • Protein expression and function analysis
样本类型以及上样量
Tests20 Colorimetric/Fluorescent
制备和贮存
保存方式
4°C
数据库链接
EU_Commodity_Code
38220000
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