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Influenza A H3N2 (A/Wyoming/03/2003) Hemagglutinin / HA Protein (His Tag)
Influenza A H3N2 (A/Wyoming/03/2003) Hemagglutinin / HA Protein (His Tag)
品牌： Sino Biological
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对比
咨询分子量：
The secreted recombinant influenza A H3N2 HA (A/Wyoming/03/2003 (H3N2)) comprises 525 amino acids and has a predicted molecular mass of 59 kDa. As a result of glycosylation, it migrates as an approximately 80-90 kDa band in SDS-PAGE under reducing conditions.
纯度：
> 90 % as determined by SDS-PAGE.
产品介绍
产品介绍
产品信息
抗原名称
Hemagglutinin
N端序列分析
Gln 17
分子量
The secreted recombinant influenza A H3N2 HA (A/Wyoming/03/2003 (H3N2)) comprises 525 amino acids and has a predicted molecular mass of 59 kDa. As a result of glycosylation, it migrates as an approximately 80-90 kDa band in SDS-PAGE under reducing conditions.
生物活性
Measured by its ability to agglutinate guinea pig red blood cells.HA titer is 0.01-0.1 μg/mL for 1% GRBC.
纯度
> 90 % as determined by SDS-PAGE.
内毒素水平
< 1.0 EU per μg of the protein as determined by the LAL method
形式
Lyophilized from sterile PBS, pH 7.4.
Please contact us for any concerns or special requirements.
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
表达系统
HEK293
应用
反应种属
H3N2
背景
背景
The influenza viral Hemagglutinin (HA) protein is a homotrimer with a receptor binding pocket on the globular head of each monomer.HA has at least 18 different antigens. These subtypes are named H1 through H18.HA has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding to these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of the host endosomal membrane with the viral membrane. The influenza virus Hemagglutinin (HA) protein is translated in cells as a single protein, HA, or hemagglutinin precursor protein. For viral activation, hemagglutinin precursor protein (HA) must be cleaved by a trypsin-like serine endoprotease at a specific site, normally coded for by a single basic amino acid (usually arginine) between the HA1 and HA2 domains of the protein. After cleavage, the two disulfide-bonded protein domains produce the mature form of the protein subunits as a prerequisite for the conformational change necessary for fusion and hence viral infectivity.
Full Name
Hemagglutinin
References
White JM, Hoffman LR, Arevalo JH, et al. Attachment and entry of influenza virus into host cells. Pivotal roles of hemagglutinin. In Chiu W, Burnett RM, Garcea RL. Structural Biology of Viruses.1997
Suzuki Y.Sialobiology of influenza: molecular mechanism of host range variation of influenza viruses. Biol. Pharm. Bull. 2005.
Senne DA, Panigrahy B, Kawaoka Y, et al. Survey of the hemagglutinin (HA) cleavage site sequence of H5 and H7 avian influenza viruses: amino acid sequence at the HA cleavage site as a marker of pathogenicity potential. Avian Dis. 1996
Donald J. Benton,Influenza hemagglutinin membrane anchor,PNAS,2018
制备和贮存
保存方式
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
数据库链接
Accession
ABX10525.1
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