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Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human insulin. Antibodies are purified by protein A and peptide affinity chromatography.
Product Usage Information
| Application | Dilution |
|---|---|
| Immunohistochemistry (Paraffin) | 1:100 |
| Immunofluorescence (Frozen) | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:100 |
| Flow Cytometry (Fixed/Permeabilized) | 1:100 |
Specificity/Sensitivity
Species Reactivity:
Human, Mouse, Rat
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
参考图片
Flow cytometric analysis of untreated β-TC cells, using Insulin Antibody (blue) compared to a nonspecific negative control antibody (red).
Immunohistochemical analysis of paraffin-embedded rat pancreas, showing staining of β-cells using Insulin Antibody.
Immunohistochemical analysis of paraffin-embedded mouse pancreas, showing staining of β cells, using Insulin Antibody.
Immunohistochemical analysis of paraffin-embedded human pancreas, showing staining of β cells, using Insulin Antibody.
Confocal immunofluorescent analysis of normal mouse pancreas using Insulin Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Confocal immunofluorescent analysis of beta-TC-6 and HeLa cells using Insulin Antibody (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).