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IRF-3 (D6I4C) XP ®  Rabbit mAb
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IRF-3 (D6I4C) XP ® Rabbit mAb

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分子量:
50-55
反应种属:
Human,Monkey,
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产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
IRF-3
来源纯化

Monoclonal antibody is produced by immunizing animals with recombinant human IRF-3 protein.

宿主
Rabbit
商品描述

Product Usage Information

ApplicationDilution
Western Blotting1:1000
Simple Western™1:50 - 1:250
Immunoprecipitation1:50
Immunofluorescence (Immunocytochemistry)1:200 - 1:800
同种型
Rabbit IgG
分子量
50-55
研究领域
免疫学和肿瘤学,神经科学,
应用
反应种属
Human,Monkey,
目标/特异性

Specificity/Sensitivity

IRF-3 (D6I4C) XP Rabbit mAb recognizes endogenous levels of total IRF-3 protein.

Species Reactivity:

Human, Monkey

敏感性
Endogenous
背景
背景
Interferon regulatory factors (IRFs) comprise a family of transcription factors that function within the Jak/Stat pathway to regulate interferon (IFN) and IFN-inducible gene expression in response to viral infection (1). IRFs play an important role in pathogen defense, autoimmunity, lymphocyte development, cell growth, and susceptibility to transformation. The IRF family includes nine members: IRF-1, IRF-2, IRF-9/ISGF3γ, IRF-3, IRF-4 (Pip/LSIRF/ICSAT), IRF-5, IRF-6, IRF-7, and IRF-8/ICSBP. All IRF proteins share homology in their amino-terminal DNA-binding domains. IRF family members regulate transcription through interactions with proteins that share similar DNA-binding motifs, such as IFN-stimulated response elements (ISRE), IFN consensus sequences (ICS), and IFN regulatory elements (IRF-E) (2).IRF-3 can inhibit cell growth and plays a critical role in controlling the expression of genes in the innate immune response (1-4). In unstimulated cells, IRF-3 is present in the cytoplasm. Viral infection results in phosphorylation of IRF-3 and leads to its translocation to the nucleus where it activates promoters containing IRF-3-binding sites. Phosphorylation of IRF-3 occurs at a cluster of C-terminal Ser and Thr residues (between 385 and 405), leading to its association with the p300/CBP coactivator protein that promotes DNA binding and transcriptional activity (5). During infection, IRF-3 is likely activated through a pathway that includes activation of Toll-like receptors and a kinase complex that includes IKKε and TBK1 (6,7). IRF-3 is phosphorylated at Ser396 following viral infection, expression of viral nucleocapsid, and double-stranded RNA treatment. These events likely play a role in activation of IRF-3 (8). 1.Taniguchi, T. et al. (2001) Annu Rev Immunol 19, 623-55. 2.Honda, K. and Taniguchi, T. (2006) Nat Rev Immunol 6, 644-58. 3.Hiscott, J. et al. (1999) J Interferon Cytokine Res 19, 1-13. 4.Kim, T.Y. et al. (2003) J Biol Chem 278, 15272-8. 5.Yoneyama, M. et al. (2002) J Interferon Cytokine Res 22, 73-6. 6.Fitzgerald, K.A. et al. (2003) Nat Immunol 4, 491-6. 7.Kopp, E. and Medzhitov, R. (2003) Curr Opin Immunol 15, 396-401. 8.Servant, M.J. et al. (2003) J Biol Chem 278, 9441-7.
研究领域
免疫学和肿瘤学,神经科学,
翻译后修饰
unmodified
制备和贮存
保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

数据库链接
Entrez-Gene ID
3661
UniProt ID
Q14653

参考图片

Confocal immunofluorescent analysis of HT-29 cells, untransfected (left) or transfected with Poly(I:C) (2.5 μg/ml, 6 hr; right), using IRF-3 (D6I4C) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western blot analysis of extracts from control HeLa cells (lane 1) or IRF-3 knockout HeLa cells (lane 2) using IRF-3 (D6I4C) XP® Rabbit mAb, #11904 (upper) or β-actin (13E5) Rabbit mAb, #4970 (lower). The absence of signal in the IRF-3 knockout HeLa cells confirms specificity of the antibody for IRF-3.

Western blot analysis of extracts from various cell lines using IRF-3 (D6I4C) XP® Rabbit mAb.

Simple Western™ analysis of lysates (1 mg/mL) from HeLa cells using IRF-3 (D6I4C) XP ® Rabbit mAb #11904. The virtual lane view (left) shows the target band (as indicated) at 1:50 and 1:250 dilutions of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at 1:50 (blue line) and 1:250 (green line) dilutions of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a BioTechne brand, using the 12-230 kDa separation module.

Immunoprecipitation of IRF-3 from THP-1 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or IRF-3 (D6I4C) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using IRF-3 (D6I4C) XP® Rabbit mAb. Mouse Anti-rabbit IgG (Conformation Specific) (L27A9) mAb #3678 and Anti-mouse IgG, HRP-linked Antibody #7076 were used as secondary antibodies.

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货号:
11904S
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