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ISRE Reporter BioAssay™ Kit (JAK/STAT Signaling Pathway)
品牌： Usbiological
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咨询来源宿主：
HEK293
实验应用：
Monitor IFNa-induced JAK/STAT pathway activity. Screen activators or inhibitors of the JAK/STAT signaling pathway. Study effects of RNAi or gene overexpression on the activity of the JAK/STAT pathway.
产品介绍
产品信息
来源纯化
HEK293
宿主
HEK293
产品用途
Monitor IFNa-induced JAK/STAT pathway activity. Screen activators or inhibitors of the JAK/STAT signaling pathway. Study effects of RNAi or gene overexpression on the activity of the JAK/STAT pathway.
简单描述
The ISRE Reporter BioAssay™ kit is designed for monitoring the activity of Type I interferon-induced JAK/STAT signaling pathway in the cultured cells. The kit contains transfection-ready ISRE luciferase reporter vector, which is a JAK/STATpathway-responsive reporter. This reporter contains a firefly luciferase gene under the control of multimerized ISRE responsive element located upstream of a minimal promoter. The ISRE reporter is premixed with constitutively-expressing Renilla luciferase vector, which serves as an internal control for transfection efficiency. The kit also includes a non-inducible firefly luciferase vector premixed with constitutively-expressing Renilla luciferase vector as a negative control. The non-inducible luciferase vector contains a firefly luciferase gene under the control of a minimal promoter, without any additional response elements. The negative control is critical to determining pathway specific effects and background luciferase activity.
应用
实验应用
Monitor IFNa-induced JAK/STAT pathway activity. Screen activators or inhibitors of the JAK/STAT signaling pathway. Study effects of RNAi or gene overexpression on the activity of the JAK/STAT pathway.
样本类型以及上样量
Tests500 Luminescent Cultured cells
制备和贮存
保存方式
-20°C/-70°C
数据库链接
EU_Commodity_Code
38220000
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