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mIntegrin b1 MAb (Cl (25 ug)
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mIntegrin b1 MAb (Cl (25 ug)

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品牌: R&D Systems
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来源宿主:
Rat IgG2a
实验应用:
Western Blot(1 µg/mL)
Flow Cytometry(0.25 µg/106 cells)
Immunohistochemistry(8-25 µg/mL)
Immunocytochemistry(8-25 µg/mL)
产品介绍
产品介绍
产品信息
荧光素标记
纯化方式
Protein A or G purified from hybridoma culture supernatant
抗原名称
CD29 antigen, CD29, Fibronectin receptor subunit beta, FNRBVLAB, GPIIA, Integrin beta 1, integrin be
宿主
Rat IgG2a
免疫原
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 1/CD29
Gln21-Asp728
Accession # P09055
克隆号
265917

Scientific Data

Flow Cytometry Detection of Integrin  beta 1/CD29 antibody in B16-F1 Mouse Cell Line antibody by Flow Cytometry.View Larger

Detection of Integrin beta 1/CD29 in B16‑F1 Mouse Cell Line by Flow Cytometry. B16-F1 mouse melanoma cell line was stained with Rat Anti-Mouse Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB2405, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Immunocytochemistry Integrin  beta 1/CD29 antibody in L-929 Mouse Cell Line by Immunocytochemistry (ICC).View Larger

Integrin beta 1/CD29 in L‑929 Mouse Cell Line. Integrin beta 1/CD29 was detected in immersion fixed L-929 mouse fibroblast cell line using Rat Anti-Mouse Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB2405) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Mouse Integrin  beta 1/CD29 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin beta 1/CD29 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Integrin  beta 1, recombinant mouse (rm) Integrin  beta 2, rmIntegrin  beta 3, rmIntegrin  beta 4, or rmIntegrin  beta 6 is observed.
Source
Monoclonal Rat IgG2A Clone # 265917
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 1/CD29
Gln21-Asp728
Accession # P09055
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Integrin  beta 1/CD29 under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
Perfusion fixed frozen sections of mouse spinal cord
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

组成成分
IGG-免疫球蛋白G型抗体
应用
实验应用
Western Blot(1 µg/mL)
Flow Cytometry(0.25 µg/106 cells)
Immunohistochemistry(8-25 µg/mL)
Immunocytochemistry(8-25 µg/mL)
背景
别名
CD29 antigen,CD29,Fibronectin receptor subunit beta,FNRBVLAB,GPIIA,Integrin beta 1,integrin beta-1,integrin VLA-4 beta subunit,integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12),ITGB1,MDF2,MSK12,very late activation protein, beta polypeptide,VLA-4 subunit beta,VLA-BETA
背景

Background: Integrin beta 1/CD29

The Integrin beta 1 subunit, also known as CD29, associates with multiple distinct Integrin alpha subunits. The Integrin beta 1 subunit has a broad tissue distribution with the exception of erythrocytes.

Entrez Gene IDs
3688 (Human); 16412 (Mouse); 102144610 (Cynomolgus Monkey)
Alternate Names
CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA
制备和贮存
溶解方法
Reconstitute at 0.5 mg/mL in sterile PBS.
保存方式

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

参考图片

Detection of Integrin  beta 1/CD29 in B16‑F1 Mouse Cell Line by Flow Cytometry. B16‑F1 mouse melanoma cell line was stained with Rat Anti-Mouse Integrin  beta 1/CD29 Monoclonal Antibody (Catalog # MAB2405, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody
(Catalog # F0105B).

Integrin  beta 1/CD29 in L‑929 Mouse Cell Line. Integrin  beta 1/CD29 was detected in immersion fixed L‑929 mouse fibroblast cell line using Rat Anti-Mouse Integrin  beta 1/CD29 Monoclonal Antibody (Catalog # MAB2405) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

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货号:
MAB2405-SP
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