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Mouse Integrin beta 1/CD29 MAb (Clone 265917) (25 ug)
Mouse Integrin beta 1/CD29 MAb (Clone 265917) (25 ug)
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Mouse Integrin beta 1/CD29 MAb (Clone 265917) (25 ug)

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来源宿主：
Rat IgG2a
实验应用：
Western Blot(1 µg/mL)
Flow Cytometry(0.25 µg/106 cells)
Immunohistochemistry(8-25 µg/mL)
Immunocytochemistry(8-25 µg/mL)
产品介绍
产品介绍
产品信息
荧光素标记
Unconjugated
纯化方式
Protein A or G purified from hybridoma culture supernatant
抗原名称
CD29 antigen， CD29， Fibronectin receptor subunit beta， FNRBVLAB， GPIIA， Integrin beta 1， integrin be
宿主
Rat IgG2a
免疫原
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 1/CD29
Gln21-Asp728
Accession # P09055
克隆号
265917
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Integrin  beta 1/CD29 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin beta 1/CD29 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human Integrin  beta 1, recombinant mouse (rm) Integrin  beta 2, rmIntegrin  beta 3, rmIntegrin  beta 4, or rmIntegrin  beta 6 is observed.
Source
Monoclonal Rat IgG2A Clone # 265917
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 1/CD29
Gln21-Asp728
Accession # P09055
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Integrin  beta 1/CD29 under non-reducing conditions only
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
Perfusion fixed frozen sections of mouse spinal cord
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Integrin beta 1/CD29 antibody in B16-F1 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Integrin beta 1/CD29 in B16‑F1 Mouse Cell Line by Flow Cytometry. B16-F1 mouse melanoma cell line was stained with Rat Anti-Mouse Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB2405, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).

Immunocytochemistry Integrin beta 1/CD29 antibody in L-929 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

Integrin beta 1/CD29 in L‑929 Mouse Cell Line. Integrin beta 1/CD29 was detected in immersion fixed L-929 mouse fibroblast cell line using Rat Anti-Mouse Integrin beta 1/CD29 Monoclonal Antibody (Catalog # MAB2405) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (yellow; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 1/CD29

The Integrin beta 1 subunit, also known as CD29, associates with multiple distinct Integrin alpha subunits. The Integrin beta 1 subunit has a broad tissue distribution with the exception of erythrocytes.

Entrez Gene IDs
3688 (Human); 16412 (Mouse); 102144610 (Cynomolgus Monkey)
Alternate Names
CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA

Product Datasheets

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Product Datasheet
COA
SDS
应用
实验应用
Western Blot(1 µg/mL)
Flow Cytometry(0.25 µg/106 cells)
Immunohistochemistry(8-25 µg/mL)
Immunocytochemistry(8-25 µg/mL)
背景
别名
CD29 antigen,CD29,Fibronectin receptor subunit beta,FNRBVLAB,GPIIA,Integrin beta 1,integrin beta-1,integrin VLA-4 beta subunit,integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12),ITGB1,MDF2,MSK12,very late activation protein, beta polypeptide,VLA-4 subunit beta,VLA-BETA
背景

Background: Integrin beta 1/CD29

The Integrin beta 1 subunit, also known as CD29, associates with multiple distinct Integrin alpha subunits. The Integrin beta 1 subunit has a broad tissue distribution with the exception of erythrocytes.

Entrez Gene IDs
3688 (Human); 16412 (Mouse); 102144610 (Cynomolgus Monkey)
Alternate Names
CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA
研究领域
制备和贮存
溶解方法
Reconstitute at 0.5 mg/mL in sterile PBS.
保存方式

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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货号：
MAB2405-SP
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