首页抗体一抗一抗

Jagged1 (D4Y1R) XP ® Rabbit mAb

1/7

Jagged1 (D4Y1R) XP ® Rabbit mAb

品牌： CST

下载产品说明书

下载COA

下载SDS

用小程序，查商品更便捷

对比

咨询

参数

分子量：

反应种属：

展开

产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

Jagged1

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1131 of human Jagged1 protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:200
Immunohistochemistry (Paraffin)	1:200
Flow Cytometry (Fixed/Permeabilized)	1:50

同种型

Rabbit IgG

分子量

180

内毒素水平

大鼠, 仓鼠

研究领域

癌症,发育生物学与干细胞研究,神经科学

应用

反应种属

Human,Mouse,Monkey,

预测反应种属

Rat,Hamster,

目标/特异性

Specificity/Sensitivity

Jagged1 (D4Y1R) XP Rabbit mAb recognizes endogenous levels of total Jagged1 protein. Based on sequence analyses, this antibody is not predicted to detect Jagged2 protein.

Species Reactivity:

Human, Mouse, Monkey

敏感性

Endogenous

背景

Notch signaling is activated upon engagement of the Notch receptor with its ligands, the DSL (Delta, Serrate, Lag2) proteins of single-pass type I membrane proteins. The DSL proteins contain multiple EGF-like repeats and a DSL domain that is required for binding to Notch (1,2). Five DSL proteins have been identified in mammals: Jagged1, Jagged2, Delta-like (DLL) 1, 3 and 4 (3). Ligand binding to the Notch receptor results in two sequential proteolytic cleavages of the receptor by the ADAM protease and the γ-secretase complex. The intracellular domain of Notch is released and then translocates to the nucleus where it activates transcription. Notch ligands may also be processed in a way similar to Notch, suggesting a bi-directional signaling through receptor-ligand interactions (4-6). 1.Wilson, A. and Radtke, F. (2006) FEBS Lett. 580, 2860-2868. 2.Hansson, E.M. et al. (2004) Semin. Cancer Biol. 14, 320-328. 3.Chiba, S. (2006) Stem Cells 24, 2437-2447. 4.Bland, C.E. et al. (2003) J. Biol. Chem. 278, 13607-13610. 5.Six, E. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 7638-7643. 6.LaVoie, M.J. and Selkoe, D.J. (2003) J. Biol. Chem. 278, 34427-34437.

研究领域

癌症,发育生物学与干细胞研究,神经科学

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #15269.

数据库链接

Entrez-Gene ID

182

UniProt ID

P78504

参考图片

Flow cytometric analysis of fixed and permeabilized Jurkat cells (blue, negative) and MKN-45 cells (green, positive) using Jagged1 (D4Y1R) XP® Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Western blot analysis of extracts from various cell lines using Jagged1 (D4Y1R) XP® Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Molt4 and Jurkat cells do not expression Jagged1 protein, confirming specificity of the antibody for Jagged1.

Immunoprecipitation of Jagged1 protein from HT-29 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) XP® IgG Isotype Control #3900, and lane 3 is Jagged1 (D4Y1R) XP® Rabbit mAb. Western blot analysis was performed using Jagged1 (D4Y1R) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded HepG2 (positive, left) and Jurkat (negative, right) cell pellets using Jagged1 (D4Y1R) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jagged1 (D4Y1R) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using Jagged1 (D4Y1R) XP(R) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

Immunohistochemical analysis of paraffin-embedded human tonsil using Jagged1 (D4Y1R) XP(R) Rabbit mAb.

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

70109T

一键复制

询价

供应商现货

20ul

100ul

选择数量

当前规格1件起购

配送至

预计5-6个工作日送达，快递: 免运费，若需干冰额外收费

询价大包装询价