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Phospho-Jak1 (Tyr1034/1035)/Jak2 (Tyr1007/1008) (E9Y7V) Mouse mAb

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Phospho-Jak1 (Tyr1034/1035)/Jak2 (Tyr1007/1008) (E9Y7V) Mouse mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1007/Tyr1008 of human Jak2 protein.

宿主

Mouse

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100

同种型

Mouse IgG2a

分子量

125, 130

应用

目标/特异性

Specificity/Sensitivity

Phospho-Jak1 (Tyr1034/1035)/Jak2 (Tyr1007/1008) (E9Y7V) Mouse mAb recognizes endogenous levels of Jak2 protein only when phosphorylated at Tyr1007 and Tyr1008 and endogenous levels of Jak1 when phosphorylated at Tyr1034 and Tyr1035. This antibody may cross-react with phospho-Jak3 and phospho-Tyk2.

Species Reactivity:

Human, Mouse

敏感性

Endogenous

背景

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains. 1.Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322. 2.Darnell, J.E. (1997) Science 277, 1630-5. 3.VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93. 4.Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92. 5.Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.

研究领域

免疫学和肿瘤学,神经科学,

翻译后修饰

phosphate

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

3717

UniProt ID

O60674

参考图片

Immunoprecipitation of Phospho-Jak2 (Tyr1007/1008) from BaF3 cell extracts treated with mouse IL-3 (10ng/ml, 5 min). Lane 1 is 10% input, lane 2 is Mouse (G3A1) mAb IgG1 Isotype Control #5415, and lane 3 is Phospho-Jak1 (Tyr1034/1035)/Jak2 (Tyr1007/1008) (E9Y7V) Mouse mAb. Western blot analysis was performed using Phospho-Jak1 (Tyr1034/1035)/Jak2 (Tyr1007/1008) (E9Y7V) Mouse mAb.

Western blot analysis of extracts from BaF3 cells, untreated (-) or treated with mouse IL-3 (10ng/ml, 5 min; +), using Phospho-Jak1 (Try1034/1035)/Jak2 (Tyr1007/1008) (E9Y7V) Mouse mAb (upper), Jak2 (D2E12) XP® Rabbit mAb #3230 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot analysis of extracts from U266 cells, untreated (-) or treated with IFNα (50ng/ml, 15 min; +), using Phospho-Jak1 (Try1034/1035)/Jak2 (Tyr1007/1008) (E9Y7V) Mouse mAb (upper), Jak1 (6G4) Rabbit mAb #3344 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower).

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货号：

66245S

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供应商现货

20μl

100ul

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