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Jak2 (D2E12) XP ® Rabbit mAb

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Jak2 (D2E12) XP ® Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

Jak2

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro841 of Jak2.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100
Immunohistochemistry (Paraffin)	1:800 - 1:3200

同种型

Rabbit IgG

分子量

125

内毒素水平

仓鼠 , 猴, 鸡 , 非洲爪蟾蜍, 牛 , 犬 , 猪 , 马

研究领域

免疫学和肿瘤学,神经科学,

应用

反应种属

Human,Mouse,Rat,

预测反应种属

Hamster,Monkey,Chicken,Xenopus,Bovine,Dog,Pig,Horse,

目标/特异性

Specificity/Sensitivity

Jak2 (D2E12) XP Rabbit mAb detects endogenous levels of total Jak2 protein. No cross-reactivity was observed with other family members.

Species Reactivity:

Human, Mouse, Rat

敏感性

Endogenous

背景

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains. 1.Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322. 2.Darnell, J.E. (1997) Science 277, 1630-5. 3.VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93. 4.Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92. 5.Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.

研究领域

免疫学和肿瘤学,神经科学,

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #34315.

数据库链接

Entrez-Gene ID

3717

UniProt ID

O60674

参考图片

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jak2 (D2E12) XP® Rabbit mA in the presence of control peptide (left) or Jak2 Blocking Peptide #1039 (right).

Western blot analysis of extracts from various cell lines using Jak2 (D2E12) XP® Rabbit mAb #3230 (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

Western blot analysis of extracts from K-562 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or Jak2 siRNA (+), using Jak2 (D2E12) XP® Rabbit mAb #3230 and α-Tubulin (11H10) Rabbit mAb #2125. The Jak2 (D2E12) XP® Rabbit mAb confirms silencing of Jak2 expression, while the α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Jak2 siRNA.

Western blot analysis of extracts from K-562, THP-1, TF-1 and BaF3 cell lines using Jak2 (D2E12) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jak2 (D2E12) XP® Rabbit mAb.

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货号：

3230T

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