JARID2 (D6M9X) Rabbit mAb

Jumonji/ARID domain-containing protein 2 (JARID2) is a founding member of the JmjC-domain-containing protein family that is involved in regulation of histone methyltransferase activity (1,2). While many proteins in this family are protein demethylases, JARID2 lacks several conserved residues in the catalytic domain and does not exhibit protein demethylase activity (1,2). Research studies indicate that JARID2 is a nuclear protein that is highly expressed in poorly differentiated and actively dividing cells, with expression decreasing upon cellular differentiation (3,4). Expression of JARID2 protein is essential for embryonic development as the protein plays an important role in regulation of heart and liver development, neural tube fusion, and hematopoiesis (4). JARID2 is an accessory component of the polycomb repressor complex 2 (PRC2), which represses target gene expression through methylation of histone H3 at lysine 27 by EZH2 methyltransferase (5-10). JARID2 recruits the PRC2 complex to target genes and increases EZH2 methyltransferase activity by binding to nucleosomes and DNA (5-10). Additional studies show that loss of JARID2 expression results in decreased recruitment of PRC2, decreased methylation of histone H3 at lysine 27 at target genes, and delayed and incomplete differentiation of embryonic stem cells (5-10). Experimental knockdown of JARID2 in Xenopus laevis impairs the induction of gastrulation genes in blastula embryos and results in differentiation failure (5). 1.Cloos, P.A. et al. (2008) Genes Dev 22, 1115-40. 2.Klose, R.J. et al. (2006) Nat Rev Genet 7, 715-27. 3.Luzzani, C. et al. (2011) Biochem Biophys Res Commun 410, 816-22. 4.Jung, J. et al. (2005) Dev Dyn 232, 21-32. 5.Peng, J.C. et al. (2009) Cell 139, 1290-302. 6.Shen, X. et al. (2009) Cell 139, 1303-14. 7.Pasini, D. et al. (2010) Nature 464, 306-10. 8.Li, G. et al. (2010) Genes Dev 24, 368-80. 9.Son, J. et al. (2013) Genes Dev 27, 2663-77. 10.Kaneko, S. et al. (2014) Mol Cell 53, 290-300.