Keratin 14 (E7W6V) Rabbit mAb (BSA and Azide Free)

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Keratin 14 (E7W6V) Rabbit mAb (BSA and Azide Free)

品牌： CST

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来源宿主：

产品介绍

产品信息

荧光素标记

Unconjugated

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human keratin 14 protein.

宿主

Rabbit

商品描述

Product Usage Information

This product is the carrier free version of product #74956. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.

This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.

BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

同种型

Rabbit IgG

应用

目标/特异性

Specificity/Sensitivity

Keratin 14 (E7W6V) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total keratin 14 protein. Non-specific staining was observed in mouse testis and stomach by immunohistochemistry.

Species Reactivity:

Human, Mouse

敏感性

Endogenous

背景

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins K9-K28) and a basic keratin (or type II keratin, keratins K1-K8 and K71-K80) assemble to form filaments. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as research and clinical biomarkers (1,2).Dysregulation/mutations in keratin genes can lead to a variety of disorders affecting the skin, hair, nails, and other epithelial tissues (3). While expression of keratins can be variable, immunohistochemical staining of keratins is widely used to help in the identification and classification of epithelial tumors, and may also provide prognostic information.Keratins 8 and 18 (K8/K18) are expressed in simple epithelia of normal tissue, as well as in adenocarcinomas of the breast, lung, ovary, and gastrointestinal tract. Keratin 17 is expressed in basal keratinocytes of stratified epithelia, hair follicles, and sebaceous glands. Onset of keratin 17 expression coincides with the definition of major epithelial lineages during skin development (4). Keratin 14 (K14) is expressed in basal cells of stratified epithelia, and in basal-like subtypes of breast cancer and squamous cell carcinomas. Keratin 19 (K19) is expressed in glandular epithelia, including the liver, gallbladder, and pancreas, as well as in adenocarcinomas of the breast, thyroid, and bile duct. Keratin 20 (K20) is expressed in gastrointestinal epithelium, urothelium, and Merkel cells in the skin, as well as in colorectal carcinomas and some urothelial carcinomas. Keratin 5/6 (K5/6) is expressed in basal cells of stratified epithelia, including the skin, prostate, and breast, as well as in basal-like breast cancers, squamous cell carcinomas, and some lung carcinomas. Keratin 7 (K7) is expressed in glandular epithelia, such as those in the lung, breast, and female reproductive tract, as well as in adenocarcinomas of the lung, breast, and ovary (5,6).Keratins, particularly K8, K18, and K19, serve as biomarkers for identification of circulating tumor cells (CTCs) (5).Post-translational modifications, including phosphorylation, acetylation, ubiquitylation, sumoylation, glycosylation, and transamidation, have been shown to affect the functions of keratins in normal and disease states (6). Understanding the molecular mechanisms underlying these PTMs may provide insights into cancer pathogenesis. 1.Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13. 2.Schweizer, J. et al. (2006) J Cell Biol 174, 169-74. 3.Sarma, A. (2022) Int J Biol Macromol 219, 395-413. 4.McGowan, K.M. and Coulombe, P.A. (1998) J Cell Biol 143, 469-86. 5.Werner, S. et al. (2020) Mol Aspects Med 72, 100817. 6.Dmello, C. et al. (2019) J Biosci 44, 33.

翻译后修饰

unmodified

制备和贮存

保存方式

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

数据库链接

Entrez-Gene ID

3861

UniProt ID

P02533

参考图片

Flow cytometric analysis of PANC-1 cells (blue, negative) and A-431 cells (green, positive) using Keratin 14 (E7W6V) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody. Data were generated using the standard formulation of this product.

Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using Keratin 14 (E7W6V) Rabbit mAb. Data were generated using the standard formulation of this product.

Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the cervix using Keratin 14 (E7W6V) Rabbit mAb. Data were generated using the standard formulation of this product.

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using Keratin 14 (E7W6V) Rabbit mAb. Data were generated using the standard formulation of this product.

Immunohistochemical analysis of paraffin-embedded normal human esophagus using Keratin 14 (E7W6V) Rabbit mAb. Data were generated using the standard formulation of this product.

Immunohistochemical analysis of paraffin-embedded human follicular hyperplasia of the tonsil using Keratin 14 (E7W6V) Rabbit mAb. Data were generated using the standard formulation of this product.

Immunohistochemical analysis of paraffin-embedded 4T1 syngeneic tumor using Keratin 14 (E7W6V) Rabbit mAb. Data were generated using the standard formulation of this product.

Immunohistochemical analysis of paraffin-embedded mouse prostate using Keratin 14 (E7W6V) Rabbit mAb. Data were generated using the standard formulation of this product.

Immunohistochemical analysis of paraffin-embedded mouse thymus using Keratin 14 (E7W6V) Rabbit mAb. Data were generated using the standard formulation of this product.

Immunohistochemical analysis of paraffin-embedded human oropharyngeal squamous cell carcinoma using Keratin 14 (E7W6V) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right). Data were generated using the standard formulation of this product.

Immunohistochemical analysis of paraffin-embedded A-431 cell pellet (left, positive) or A-673 cell pellet (right, negative) using Keratin 14 (E7W6V) Rabbit mAb. Data were generated using the standard formulation of this product.

Confocal immunofluorescent analysis of fixed frozen mouse skin labeled with Keratin 14 (E7W6V) Rabbit mAb (left, green). Free secondary binding sites were then blocked with Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 prior to co-labeling with Vimentin (D21H3) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #9855 (right, red) and ProLong Gold Antifade Reagent with DAPI #8961 (right, blue). Data were generated using the standard formulation of this product.

Confocal immunofluorescent analysis of A-431 cells (left, positive) and A-673 cells (right, negative) using Keratin 14 (E7W6V) Rabbit mAb (green), DyLight 554 Phalloidin #13054 (red), and DAPI #4083 (blue). Data were generated using the standard formulation of this product.

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货号：

95052SF

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询价

100μg

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