BD OptiBuild™ BV510 Hamster Anti-Mouse KLRG1

KLRG1

Syrian Hamster IgG2, κ

A-LAK from C57BL/6 mice

The 2F1 monoclonal antibody specifically binds to KLRG1 ( K iller cell L ectin-like R eceptor G1 ), which is the mouse homolog of the rat mast cell function-associated antigen (MAFA), on all mouse strains tested (eg, AKR/J, BALB/c, C3H/HeN, C3H.SW, C57BL/6, DBA/1, SJL, 129/J). Unlike rat MAFA, which is expressed on mast cells, mouse KLRG1 is expressed on a large subset of NK cells, lymphokine-activated killer (LAK) cells, adherent LAK (A-LAK) cells, subsets of activated CD8+ T lymphocytes, and small fractions of CD4+ and CD8+ T cells, but not mast cells. The expression of KLRG1 is correlated with reduced proliferative capacity of activated T lymphocytes or reduced effector functions of activated NK cells. KLRG1 plays a role in the regulation of leucocytes of both the innate and adaptive immune system. The 2F1 mAb reportedly stains the rat basophilic leukemia cell line, RBL-2H3, which is known to express MAFA. The KLRG1 protein is an inhibitory lectin-like type II transmembrane receptor containing a cytoplasmic motif similar to ITIM ( I mmunoreceptor T yrosine-based I nhibitory M otif); its ligand has not been identified KLRG1 is expressed mainly as a homodimeric molecule consisting of two N-glycosylated subunits of approximately 30-38 kDa. The level of KLRG1 expression is reduced in MHC class I-deficient mice, although direct binding of KLRG1 to MHC class I antigens could not be detected. Crosslinking of KLRG1 by 2F1 mAb reduces TCR-mediated Ca++ mobilization and cytotoxic responses (but not IFN-γ production) by CD8+ T cells and inhibits IFN-γ and TNF production and redirected lysis by NK cells. The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.

2F1 The 2F1 monoclonal antibody specifically binds to KLRG1 ( K iller cell L ectin-like R eceptor G1 ), which is the mouse homolog of the rat mast cell function-associated antigen (MAFA), on all mouse strains tested (eg, AKR/J, BALB/c, C3H/HeN, C3H.SW, C57BL/6, DBA/1, SJL, 129/J). Unlike rat MAFA, which is expressed on mast cells, mouse KLRG1 is expressed on a large subset of NK cells, lymphokine-activated killer (LAK) cells, adherent LAK (A-LAK) cells, subsets of activated CD8+ T lymphocytes, and small fractions of CD4+ and CD8+ T cells, but not mast cells. The expression of KLRG1 is correlated with reduced proliferative capacity of activated T lymphocytes or reduced effector functions of activated NK cells. KLRG1 plays a role in the regulation of leucocytes of both the innate and adaptive immune system. The 2F1 mAb reportedly stains the rat basophilic leukemia cell line, RBL-2H3, which is known to express MAFA. The KLRG1 protein is an inhibitory lectin-like type II transmembrane receptor containing a cytoplasmic motif similar to ITIM ( I mmunoreceptor T yrosine-based I nhibitory M otif); its ligand has not been identified KLRG1 is expressed mainly as a homodimeric molecule consisting of two N-glycosylated subunits of approximately 30-38 kDa. The level of KLRG1 expression is reduced in MHC class I-deficient mice, although direct binding of KLRG1 to MHC class I antigens could not be detected. Crosslinking of KLRG1 by 2F1 mAb reduces TCR-mediated Ca++ mobilization and cytotoxic responses (but not IFN-γ production) by CD8+ T cells and inhibits IFN-γ and TNF production and redirected lysis by NK cells. The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405-nm and Em Max at 510-nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50-nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser.

Syrian Hamster IgG2, κ

克隆 2F1 (RUO)

0.2 mg/ml

Flow cytometry (Qualified)

Mouse (Tested in Development)

KLRG1

Klrg1; Killer cell lectin-like receptor subfamily G member 1; MAFA; 2F1 Ag

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(3) 1. Beyersdorf NB, Ding X, Karp K, Hanke T. Expression of inhibitory "killer cell lectin-like receptor G1" identifies unique subpopulations of effector and memory CD8 T cells. Eur J Immunol. 2001; 31(12):3443-3452. (Clone-specific: Bioassay, Blocking, Functional assay). 2. Corral L, Hanke T, Vance RE, Cado D, Raulet DH. NK cell expression of the killer cell lectin-like receptor G1 (KLRG1), the mouse homolog of MAFA, is modulated by MHC class I molecules. Eur J Immunol. 2000; 30(3):920-930. (Immunogen: Flow cytometry, Immunoprecipitation). 3. Hanke T, Corral L, Vance RE, Raulet DH. 2F1 antigen, the mouse homolog of the rat "mast cell function-associated antigen", is a lectin-like type II transmembrane receptor expressed by natural killer cells. Eur J Immunol. 1998; 28(12):4409-4417. (Clone-specific: Flow cytometry).

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