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Phospho-PKC Substrate Motif [(R/K)XpSX(R/K)] MultiMab™ Rabbit mAb mix

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Phospho-PKC Substrate Motif [(R/K)XpSX(R/K)] MultiMab™ Rabbit mAb mix

品牌： CST

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产品介绍

产品信息

抗原名称

PKC Substrate Motif

来源纯化

MultiMab

宿主

Rabbit

简单描述

Monoclonal Antibody for studying PKC Substrate (Ser) phosphate. Cited in 38 publications. Validated for WB, IP, E. Highly specific and rigorously validated in-house, Phospho-PKC Substrate Motif [(R/K)XpSX(R/K)] MultiMab® Rabbit Monoclonal Antibody mix (CST #6967) is ready to ship.

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50
Peptide ELISA (DELFIA)	1:1000

应用

反应种属

All

目标/特异性

Specificity/Sensitivity

Phospho-PKC Substrate Motif [(R/K)XpSX(R/K)] MultiMab® Rabbit mAb mix detects endogenous levels of many cellular proteins only when phosphorylated at Ser residues surrounded by Arg or Lys at the -2 and +2 positions. The antibody may also cross-react with S*X(R/K) motif-containing proteins. The antibody does not cross-react with nonphosphorylated Ser residues, phospho-Thr in the same motif, or phospho-Ser in other motifs.

Species Reactivity:

All Species Expected

敏感性

Endogenous

背景

Although protein kinase C (PKC) family members are involved in a number of signal transduction processes including secretion, gene expression, proliferation, and muscle contraction, many PKC substrates continue to be unidentified (1,2). Isozymes of PKC are subdivided into conventional PKCs (cPKC), novel PKCs (nPKC), and atypical PKCs (aPKC). PKCα, βI, βII, and γ isoforms belong to the cPKC group (1). When activated, cPKC isozymes phosphorylate substrates containing Ser or Thr, with Arg or Lys at the -3, -2, and +2 positions, and a hydrophobic amino acid at position +1 (1-3). 1.Nishikawa, K. et al. (1997) J Biol Chem 272, 952-60. 2.Pearson, R.B. and Kemp, B.E. (1991) Methods Enzymol 200, 62-81. 3.Obata, T. et al. (2000) J Biol Chem 275, 36108-15.

翻译后修饰

Phosphate

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

参考图片

Western blot analysis of extracts from Jurkat cells, untreated (-) or TPA-treated (200 nM, 30 min; +), using Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb. Western blot was imaged using Odyssey® Infrared Imaging System (LI-COR® Biotechnologies).

对未处理（-）或200nM TPA处理30分钟（+）的Jurkat细胞抽提液使用Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb进行Western blot分析。Western Blot图片采集使用Odyssey® Infrared Imaging System (LI-COR® Biotechnologies)。

Immunoprecipitation (IP) of extracts from Jurkat cells, untreated (-) or TPA-treated (200 nM, 30 min; +) (lanes 3 and 4), using Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb. 10% input is shown in lanes 1 and 2. Western blot analysis was performed using the same antibody.

对未处理（-）或200nM TPA处理30分钟的（+）（列3、4）的Jurkat细胞，使用Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb兔单抗进行免疫沉淀。10%输入对照显示在列1和2。Western Blot分析用相同抗体进行。

Western blot analysis of extracts from various mouse tissues using Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb.

对多种小鼠组织抽提液使用Phospho-(Ser) PKC Substrate (P-S3-101) Rabbit mAb兔单抗进行Western blot分析。

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货号：

6967S

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供应商现货

20μl

100ul

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