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LAMP1 (D2D11) XP ® Rabbit mAb
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Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment of human LAMP1 protein.
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:100 - 1:400 |
| Immunofluorescence (Immunocytochemistry) | 1:100 - 1:400 |
| Flow Cytometry (Fixed/Permeabilized) | 1:100 - 1:400 |
Specificity/Sensitivity
Species Reactivity:
Human, Monkey
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier-free (BSA and azide free) version of this product see product #52252.
参考图片
Flow cytometric analysis of Jurkat cells using LAMP1 (D2D11) XP® Rabbit mAb (solid line) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Western blot analysis of extracts from various cell lines using LAMP1 (D2D11) XP® Rabbit mAb.
Immunoprecipitation of LAMP1 from SNB-19 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is LAMP1 (D2D11) XP® Rabbit mAb. Western blot was performed using LAMP1 (D2D11) XP® Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
Immunohistochemical analysis of normal human kidney using LAMP1 (D2D11) XP® Rabbit mAb.
Immunohistochemical analysis of human lung carcinoma using LAMP1 (D2D11) XP® Rabbit mAb.
Immunohistochemical analysis of human ovarian serous cystadenoma using LAMP1 (D2D11) XP® Rabbit mAb.
Confocal immunofluorescent analysis of HeLa cells using LAMP1 (D2D11) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Confocal immunofluorescent analysis of human iPSC-derived cortical glutamatergic neurons at 7 days in vitro using LAMP1 (D2D11) XP® Rabbit mAb (green), β3-Tubulin (E9F3E) Mouse mAb #45058 (red), and DAPI #4083 (blue). iCell GlutaNeurons were kindly provided by FUJIFILM Cellular Dynamics, Inc.
Confocal immunofluorescent analysis of human iPSC-derived astrocyte cells at 7 days in vitro using LAMP1 (D2D11) XP® Rabbit mAb (green), GFAP (GA5) Mouse mAb #3670 (red), and DAPI #4083 (blue). iCell Astrocytes 2.0 were kindly provided by FUJIFILM Cellular Dynamics, Inc.