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LAMTOR1/C11orf59 (D11H6) XP ®  Rabbit mAb (BSA and Azide Free)
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LAMTOR1/C11orf59 (D11H6) XP ® Rabbit mAb (BSA and Azide Free)

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品牌: CST
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分子量:
18
来源宿主:
Rabbit
产品介绍
产品介绍
产品信息
荧光素标记
来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala71 of human LAMTOR1/C11orf59 protein.

宿主
Rabbit
商品描述

Product Usage Information

This product is the carrier free version of product #8975. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol.


This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.


BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity.

同种型
Rabbit IgG
分子量
18
应用
目标/特异性

Specificity/Sensitivity

LAMTOR1/C11orf59 (D11H6) XP Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total LAMTOR1/C11orf59 protein. This antibody may cross-react with LAMTOR2/ROBLD3 and LAMTOR3/MAPKSP1.

Species Reactivity:

Human, Mouse, Rat, Monkey

敏感性
Endogenous
背景
背景
mTORC1 kinase complex is a critical component in the regulation of cell growth (1,2). Its activity is modulated by energy levels, growth factors, and amino acids (3,4). The four related GTPases, RagA, RagB, RagC, and RagD, have been shown to interact with raptor in mTORC1 (1,2). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (1,2). A protein complex consisting of LAMTOR1/C11orf59, LAMTOR2/ROBLD3, and LAMTOR3/MAPKSP1 has been identified to interact with and recruit the four Rag GTPases to the surface of lysosomes (5). 1.Sancak, Y. et al. (2008) Science 320, 1496-501. 2.Kim, E. et al. (2008) Nat Cell Biol 10, 935-45. 3.Hay, N. and Sonenberg, N. (2004) Genes Dev 18, 1926-45. 4.Wullschleger, S. et al. (2006) Cell 124, 471-84. 5.Sancak, Y. et al. (2010) Cell 141, 290-303.
翻译后修饰
unmodified
制备和贮存
保存方式

Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance.

数据库链接
Entrez-Gene ID
55004
UniProt ID
Q6IAA8

参考图片

Confocal immunofluorescent analysis of HeLa cells using LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). Data were generated using the standard formulation of this product.

Western blot analysis of extracts from various cell lines using LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb. Data were generated using the standard formulation of this product.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb. Data were generated using the standard formulation of this product.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb. Data were generated using the standard formulation of this product.

Immunohistochemical analysis of paraffin-embedded mouse colon using LAMTOR1/C11orf59 (D11H6) XP® Rabbit mAb. Data were generated using the standard formulation of this product.

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货号:
87786SF
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询价
100ug
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