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Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human LARS protein.
Product Usage Information
For more information about the RBP-eCLIP service please visit Eclipsebio.
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| eCLIP | 1:200 |
Specificity/Sensitivity
Species Reactivity:
Human, Monkey
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
参考图片
Enhanced cross-linking and immunoprecipitation (eCLIP) was performed with RNA from K-562 cells and LARS (D2N4M) Rabbit mAb using a protocol based on the RBP-eCLIP method from Eclipsebio. The figure shows binding across the FOXK2 transcript. Data is kindly provided by the laboratory of Dr. Gene Yeo and used with permission.
Western blot analysis of extracts from various cell lines using LARS (D2N4M) Rabbit mAb.
Immunoprecipitation of LARS from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is LARS (D2N4M) Rabbit mAb. Western blot analysis was performed using LARS (D2N4M) Rabbit mAb.