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Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunohistochemistry (Paraffin) | 1:250 - 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:50 - 1:200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:100 - 1:400 |
Specificity/Sensitivity
物种反应性：
人, 小鼠, 大鼠
参考图片
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using LC3A/B (D3U4C) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
免疫组化分析石蜡包埋的人肺癌组织，使用的抗体是LC3A/B (D3U4C) XP® Rabbit mAb 兔单抗，分别经对照肽（左），或抗原特异性多肽（右）处理。
Immunohistochemical analysis of paraffin-embedded mouse prostate using LC3A/B (D3U4C) XP® Rabbit mAb.
免疫组化分析石蜡包埋的小鼠前列腺，使用的抗体是LC3A/B (D3U4C) XP® Rabbit mAb 兔单抗。
Confocal immunofluorescent analysis of HeLa (upper) and C2C12 (lower) cells, chloroquine-treated (50 μM, overnight; left), nutrient-starved with EBSS (3 hr, middle) or untreated (right) using LC3A/B (D3U4C) XP® Rabbit mAb (green) and β-Actin (13E5) Rabbit mAb (Alexa Fluor® 555 Conjugate) #8046 (red). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).
共聚焦免疫荧光分析HeLa（上图）和C2C12（下图）细胞，氯喹处理(50 μM, 过夜; 左)， EBSS营养饥饿处理(3 小时, 中)或未处理（右），使用的抗体是LC3A/B (D3U4C) XP® Rabbit mAb 兔单抗（绿色）和 β-Actin (13E5) Rabbit mAb (Alexa Fluor® 555 Conjugate) 兔单抗#8046（红色）。蓝色伪彩= DRAQ5® #4084（DNA荧光染料）。
Western blot analysis of extracts from RD cells, untreated (-) or Torin 1-treated (250 nM, 4 hr; +), using LC3A/B (D3U4C) XP® Rabbit mAb.
Western blot分析RD细胞的提取物，未处理(-)或 Torin 1 (托林1)处理(250 nM, 4小时; +)，使用的抗体是LC3A/B (D3U4C) XP® Rabbit mAb 兔单抗。
Flow cytometric analysis of HeLa cells, untreated (blue) or treated with chloroquine (50 µM, 16 hr) (green), using LC3A/B (D3U4C) Rabbit mAb. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.
Western blot analysis of extracts from HeLa, NIH/3T3, and KNRK cells, untreated (-) or chloroquine-treated (50 μM, overnight; +), using LC3A/B (D3U4C) XP® Rabbit mAb.
Western blot分析HeLa，NIH/3T3和KNRK细胞的提取物，未处理(-)或氯喹处理(50 μM, 过夜; +)，使用的抗体是LC3A/B (D3U4C) XP® Rabbit mAb 兔单抗。
Immunohistochemical analysis of paraffin-embedded NIH/3T3 cell pellets, control (left) or chloroquine-treated (right), using LC3A/B (D3U4C) XP® Rabbit mAb.
免疫组化分析石蜡包埋的NIH/3T3细胞沉淀，对照（左）或氯喹处理（右），使用的抗体是LC3A/B (D3U4C) XP® Rabbit mAb 兔单抗。