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Western Blot: LIF Antibody (39N7D10) [NBP2-27406] - WB validation of LIF antibody (clone 39N7D10) on (A) full-length recombinant Lif protein, (B) mouse spleen lysate and (C) human spleen lysate. 3 ug/ml concentration of primary antibody, Goat anti-rat Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this assay.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse brain using 5 ug/ml concentration of LIF antibody (clone 39N7D10).
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human prostate using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Cell surface/membrane- cytoplasmic immunepositivity of LIF was observed specifically in the epithelial cells of prostate alveolar glands whereas the surrounding fibromuscular stroma cells did not develop any staining.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - IHC analysis of formalin-fixed paraffin-embedded tissue section of normal human kidney using 5 ug/ml concentration of LIF antibody (clone 39N7D10). Expected membrane- cytoplasmic immunepositivity of LIF was observed in the cuboidal epithelial cells of renal tubules.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - IHC analysis of formalin-fixed paraffin-embedded tissue section of adenocarcinoma of human rectum using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The cancer cells as well as the goblet cells in the rectal glands depicted membrane-cytoplasmic immunostaining of LIF protein.
Immunohistochemistry-Paraffin: LIF Antibody (39N7D10) [NBP2-27406] - IHC analysis of formalin-fixed paraffin-embedded tissue section of mouse colon using 5 ug/ml concentration of LIF antibody (clone 39N7D10). The columnar epithelial cells of the crypts developed intense membrane-cytoplasmic LIF immunostaining. Additionally, some cells in the lamina propria and the sub-mucosal layer also depicted weak positivity for LIF staining.