The Mouse Lineage Panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, and erythrocytes. This Panel of five pre-diluted biotinylated antibodies is designed for the flow cytometric or immunomagnetic enrichment of hematopoietic progenitors from mouse bone marrow by depletion of cells committed to the T- and B-lymphocytic, myeloid (monocytic and granulocytic), and erythroid lineages. The Panel contains sufficient reagents to stain 10^9 bone marrow cells.
克隆号
(RUO)
BD化合物表
描述
数量/尺寸
零件号
EntrezGene ID
Biotin Hamster anti-Mouse CD3e
2 mL (1 ea)
51-01082J
N/A
Biotin Rat anti-Mouse CD11b
2 mL (1 ea)
51-01712J
N/A
Biotin Rat anti-Mouse CD45R
2 mL (1 ea)
51-01122J
N/A
Biotin Rat anti-Mouse Ly-6G and Ly-6C
2 mL (1 ea)
51-01212J
N/A
Biotin Rat anti-Mouse TER-119/Erythroid cells
2 mL (1 ea)
51-09082J
N/A
应用
实验应用
Flow cytometry (Routinely Tested)
反应种属
Mouse (QC Testing)
目标/特异性
CD3e, CD11b, CD45R, Ly-76, Ly-6G, Ly-6C
文献
文献
研发参考(6)
1. Goodell MA, Rosenzweig M, Kim H, et al. Dye efflux studies suggest that hematopoietic stem cells expressing low or undetectable levels of CD34 antigen exist in multiple species. Nat Med. 1997; 3(12):1337-1345. (Biology).
2. Morrison SJ, Wandycz AM, Hemmati HD, Wright DE, Weissman IL. Identification of a lineage of multipotent hematopoietic progenitors. Development. 1997; 124(10):1929-1939. (Biology).
3. Osawa M, Tokumoto Y, Nakauchi H. Hematopoietic stem cells. In: Herzenberg LA, Weir DM, Blackwell C, ed. Weir's Handbook of Experimental Immunology, 5th Edition. Cambridge: Blackwell Science; 1996:66.1-66.5.
4. Sato T, Laver JH, Ogawa M. Reversible expression of CD34 by murine hematopoietic stem cells. Blood. 1999; 94(8):2548-2554. (Biology).
5. Spangrude GJ, Heimfeld S, Weissman IL. Purification and characterization of mouse hematopoietic stem cells. Science. 1988; 241(4861):58-62. (Biology).
6. Spangrude GJ, Scollay R. A simplified method for enrichment of mouse hematopoietic stem cells. Exp Hematol. 1990; 18(8):920-926. (Biology).
参考图片
Depletion of lineage-committed cells from mouse bone marrow. BALB/c bone-marrow cells were labeled with BD™ Mouse Biotin Lineage Panel (Cat. No. 559971) and BD™ IMag Streptavidin Particles Plus - MSC (Cat. No. 557812, at 10 µl per 1 x 10^7 total cells). The labeled cells were then separated over a magnetic separation column designed for depletions. To demonstrate the efficiency of the depletion, unmanipulated bone-marrow cells and the lineage-negative fraction were stained with APC Rat Anti-Mouse CD117 (Cat. No. 553356) to detect hematopoietic progenitors, and with PE Rat Anti-Mouse TER-119/Erythroid Cells (Cat. No. 553673), CD45R/B220 (Cat. No. 553089/553090), and CD11b (Cat. No. 557397/553311) to detect lineage-committed cells. The lineage-negative fraction contains a greatly increased proportion of CD117+ cells and less than 5% of lineage-positive contaminants.
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