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品牌： R&D Systems
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产品信息
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Product Summary
The Parameter LTB4 Immunoassay is a 4.5 hour forward sequential competitive enzyme immunoassay designed to measure LTB4 in cell culture supernates, serum, and plasma.
Recovery
The recovery of LTB4 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 95 | 86-114 |
| EDTA Plasma (n=4) | 91 | 82-112 |
| Heparin Plasma (n=4) | 98 | 82-115 |
| Serum (n=4) | 98 | 82-118 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of LTB4 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Scientific Data
LTB4 Parameter Assay Kit Summary
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
10.9 pg/mL
Assay Range
10.3 - 2,500 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Measures LTB4 levels in various samples
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
背景
别名
Leukotriene B4,LTB4
背景
Background: Leukotriene B4/LTB4
Leukotriene B4 (LTB4) is a major product of arachidonic acid metabolism and is formed via the 5-lipoxygenase pathway. LTB4 stimulates leukocyte functions including lysosomal enzyme release, adhesion, and aggregation of polymorphonuclear leukocytes. LTB4 has been implicated as a potent mediator of inflammatory diseases and immunoregulation.
Alternate Names:
Leukotriene B4; LTB4
研究领域
制备和贮存
保存方式
Preparation and Storage
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
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