BD Pharmingen™ APC-Cy™7 Rat Anti-Mouse Ly-6G
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BD Pharmingen™ APC-Cy™7 Rat Anti-Mouse Ly-6G

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品牌: BD Pharmingen
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    反应种属:
    Mouse (QC Testing)
    Mouse (QC Testing)
    来源宿主:
    Rat LEW, also known as Lewis IgG2a, κ
    Rat LEW, also known as Lewis IgG2a, κ
    展开
    产品介绍
    产品信息
    耦联标记
    APC-Cy7
    抗原名称
    Ly-6G
    宿主
    Rat LEW, also known as Lewis IgG2a, κ
    免疫原
    Ly-6G-transfected EL4J Cell Line
    简单描述
    The 1A8 monoclonal antibody specifically binds to Ly-6G, a 21-25-kDa GPI-anchored protein. In the bone marrow, Ly6G is expressed on the majority of the largest cells, predominantly granulocytes, but not on lymphoid or erythroid cells.  In the periphery, it is expressed on granulocytes. The mAb RB6-8C5 recognizes both Ly-6G and Ly-6C and blocks the binding of mAb 1A8 to Ly-6G.
    商品描述
    1A8 The 1A8 monoclonal antibody specifically binds to Ly-6G, a 21-25-kDa GPI-anchored protein. In the bone marrow, Ly6G is expressed on the majority of the largest cells, predominantly granulocytes, but not on lymphoid or erythroid cells.  In the periphery, it is expressed on granulocytes. The mAb RB6-8C5 recognizes both Ly-6G and Ly-6C and blocks the binding of mAb 1A8 to Ly-6G.
    同种型
    Rat LEW, also known as Lewis IgG2a, κ
    克隆号
    克隆 1A8 (RUO)
    浓度
    0.2 mg/ml
    产品详情
    APC-Cy7
    APC-Cy7 dye is a part of the BD APC red family of dyes. This tandem fluorochrome is comprised of a Allophycocyanin (APC) donor that has excitation maxima (Ex Max) of 651 nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 779 nm. APC-Cy7 can be excited by the Red (627-640 nm) laser and detected using an optical filter centered near 780 nm (e.g., a 760/60 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    APC-Cy7
    Red 627-640 nm
    651 nm
    779 nm
    应用
    实验应用
    Flow cytometry (Routinely Tested)
    反应种属
    Mouse (QC Testing)
    目标/特异性
    Ly-6G
    背景
    别名
    Ly6g; Lymphocyte antigen 6G; Lymphocyte antigen 6 complex, locus G; Gr1
    制备和贮存
    存储溶液
    Aqueous buffered solution containing ≤0.09% sodium azide.
    保存方式
    Aqueous buffered solution containing ≤0.09% sodium azide.
    文献
    文献
    研发参考(1) 1. Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol. 1993; 151(5):2399-2408. (Immunogen).

    参考图片

    Analysis of Ly-6G on mouse bone marrow. Bone marrow cells from C57BL/6 mice were stained either with a APC-Cy™7 Rat IgG2a, κ isotype control ( Cat. No. 552770; unshaded histogram) or with the APC-Cy™7 Rat Anti-Mouse Ly-6G antibody (Cat. No. 560600; shaded histogram). Histograms were derived from gated events based on light scattering characteristics for bone marrow myeloid cells. Flow cytometry was performed on a BD™ LSR II flow cytometry system.

    Analysis of Ly-6G on mouse bone marrow.  Bone marrow cells from C57BL/6 mice were stained either with a APC-Cy™7 Rat IgG2a, κ isotype control (Cat. No. 552770; unshaded histogram) or with the APC-Cy™7 Rat Anti-Mouse Ly-6G antibody (Cat. No. 560600; shaded histogram).  Histograms were derived from gated events based on light scattering characteristics for bone marrow myeloid cells.  Flow cytometry was performed on a BD™ LSR II flow cytometry system.

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    货号:
    560600
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    询价
    50ug
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