The 1A8 monoclonal antibody specifically binds to Ly-6G, a 21-25-kDa GPI-anchored protein. In the bone marrow, Ly6G is expressed on the majority of the largest cells, predominantly granulocytes, but not on lymphoid or erythroid cells. In the periphery, it is expressed on granulocytes. The mAb RB6-8C5 recognizes both Ly-6G and Ly-6C and blocks the binding of mAb 1A8 to Ly-6G.
商品描述
1A8
The 1A8 monoclonal antibody specifically binds to Ly-6G, a 21-25-kDa GPI-anchored protein. In the bone marrow, Ly6G is expressed on the majority of the largest cells, predominantly granulocytes, but not on lymphoid or erythroid cells. In the periphery, it is expressed on granulocytes. The mAb RB6-8C5 recognizes both Ly-6G and Ly-6C and blocks the binding of mAb 1A8 to Ly-6G.
同种型
Rat LEW, also known as Lewis IgG2a, κ
克隆号
克隆 1A8 (RUO)
浓度
0.2 mg/ml
产品详情
PerCP-Cy5.5
PerCP-Cy5.5 dye is part of the BD blue family of dyes. This tandem fluorochrome is comprised of a fluorescent protein complex (PerCP) with an excitation maximum (Ex Max) of 482 nm and an acceptor dye with an emission maximum (Em Max) at 676 nm. PerCP-Cy5 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 680 nm (e.g., a 695/40 nm bandpass filter). The donor dye can be partially excited by the Violet (405-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
研发参考(1)
1. Fleming TJ, Fleming ML, Malek TR. Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol. 1993; 151(5):2399-2408. (Immunogen).
参考图片
Analysis of Ly-6G on mouse bone marrow. Bone marrow cells from C57BL/6 mice were stained either with a PerCP-Cy™5.5 Rat IgG2a, κ isotype control (Cat. No. 550765; unshaded) or with the PerCP-Cy™5.5 Rat Anti-Mouse Ly-6G antibody (Cat. No. 560602; shaded). Histograms were derived from gated events based on light scattering characteristics for bone marrow myeloid cells. Flow cytometry was performed on a BD™ LSR II flow cytometry system.
Analysis of Ly-6G on mouse bone marrow. Bone marrow cells from C57BL/6 mice were stained either with a PerCP-Cy™5.5 Rat IgG2a, κ isotype control (Cat. No. 550765; unshaded) or with the PerCP-Cy™5.5 Rat Anti-Mouse Ly-6G antibody (Cat. No. 560602; shaded). Histograms were derived from gated events based on light scattering characteristics for bone marrow myeloid cells. Flow cytometry was performed on a BD™ LSR II flow cytometry system.
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