BD Pharm Lyse™ Lysing Buffer

BD Pharm Lyse™ lysing solution is a buffered, concentrated (10X) ammonium chloride-based lysing reagent. When diluted to a 1X concentration and used as recommended, BD Pharm Lyse™ lyses red blood cells following monoclonal antibody staining. The lysing solution results in good light scatter separation of lymphocytes and red blood cell debris when analyzed by flow cytometry.  BD Pharm Lyse™ does not contain a fixative agent, so leukocytes remain viable after red blood cell lysis.

(RUO)

Flow cytometry (Routinely Tested)

PharM Lyse Buffer

Development References(1) 1. Muirhead KA, Wallace PK, Schmitt TC, Frescatore RL, Franco JA, Horan PK. Methodological considerations for implementation of lymphocyte subset analysis in a clinical reference laboratory. Ann N Y Acad Sci. 1986; 468:113-127. (Methodology: Flow cytometry).