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M-MLV Reverse Transcriptase
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咨询76 kDa (Reducing)
76 kDa (Reducing)
50 mM Tris-HCl、150 mM NaCl、1 mM DTT、0.1 mM EDTA、50% Glycerol、0.1% NP-40、pH 7.6 @ 25°C
Storage Solution : 200 U/ul M-MLV Reverse Transcriptase、50 mM Tris-HCl、150 mM NaCl、1 mM DTT、0.1 mM EDTA、50% Glycerol、0.1% NP-40、pH 7.6 @ 25°C
10*Reaction Buffer: 500 mM Tris-HCl、750 mM KCl、30 mM MgCl2、100 mM DTT(pH 8.3 @ 25°C)
1μg (R: reducing condition, N: non-reducing condition).
"}]Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) is an RNA-dependent DNA polymerase. The enzyme can use RNA (when synthesizing cDNA) or single-stranded DNA as a template and initiate the synthesis of a complementary DNA strand from a primer. M-MLV reverse transcriptase has no 3´ → 5´ exonuclease activity.
Store at -25 ~ -15℃ for 2 years
[1] Blain, S. W. , and S. P. Goff . "Differential Effects of Moloney Murine Leukemia Virus Reverse Transcriptase Mutations on RNase H Activity in Mg and Mn." Journal of Biological Chemistry 271.3(1996):1448-54.
[2] Narukawa, Yutaro , et al. "Improvement of Moloney murine leukemia virus reverse transcriptase thermostability by introducing a disulfide bridge in the ribonuclease H region." Protein Engineering, Design and Selection (2021).