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产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
NIK
来源纯化
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues adjacent to glycine 659 of human NIK. Antibodies are purified by protein A and peptide affinity chromatography.
宿主
Rabbit
商品描述
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
分子量
125
内毒素水平
大鼠, 猴, 牛 , 马
研究领域
免疫学和肿瘤学,神经科学,
应用
反应种属
Human,Mouse,
预测反应种属
Rat,Monkey,Bovine,Horse,
目标/特异性
Specificity/Sensitivity
NIK Antibody detects endogenous levels of total NIK protein.
Species Reactivity:
Human, Mouse
敏感性
Endogenous
背景
背景
Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11).Activation of NF-kappaB can be controlled by NF-kB-inducing kinase (NIK), a member of the MAP3K family that was originally identified as a TRAF2-interacting protein and thereby coupled to receptor activation (12). NIK forms a complex with and phosphorylates IKK1 and IKK2, subsequently leading to the phosphorylation of IkappaB and translocation of NF-kappaB to the nucleus (13-15).
1.Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
2.Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
3.Haskill, S. et al. (1991) Cell 65, 1281-9.
4.Thompson, J.E. et al. (1995) Cell 80, 573-82.
5.Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26.
6.Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7.Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63.
8.Chen, Z.J. et al. (1996) Cell 84, 853-62.
9.Senftleben, U. et al. (2001) Science 293, 1495-9.
10.Coope, H.J. et al. (2002) EMBO J 21, 5375-85.
11.Xiao, G. et al. (2001) Mol Cell 7, 401-9.
12.Malinin, N.L. et al. (1997) Nature 385, 540-4.
13.Régnier, C.H. et al. (1997) Cell 90, 373-83.
14.Woronicz, J.D. et al. (1997) Science 278, 866-9.
15.Ling, L. et al. (1998) Proc Natl Acad Sci U S A 95, 3792-7.
研究领域
免疫学和肿瘤学,神经科学,
翻译后修饰
unmodified
制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
数据库链接
Entrez-Gene ID
9020
UniProt ID
Q99558
参考图片
Western blot analysis of extacts from HeLa cells, untransfected or transfected with HA-NIK, using NIK Antibody #4994.
Western blot analysis of extracts from various cell lines, untreated or treated with 10uM MG132, using NIK Antibody #4994.
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