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对比
咨询Recovery
The recovery of mouse Adiponectin spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=7) | 103 | 82-115 |
| EDTA Plasma (n=4) | 92 | 81-106 |
| Heparin Plasma (n=4) | 93 | 77-110 |
| Serum (n=6) | 98 | 85-114 |
Linearity
Scientific Data
View LargerMouse Adiponectin/Acrp30 Quantikine ELISA Kit Changes in steroid hormone levels and adipokine levels in peripheral blood. (a) Testosterone. (b) Estradiol. (c) Corticosterone. (d) DHEA. (e) Leptin. (f) Adiponectin. For all groups, n = 8. Values are expressed as mean ± standard error. *P < 0.05, significantly different from the 3moR group. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/s41598-017-12358-8), licensed under a CC-BY license. Not internally tested by R&D Systems.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 3 hours.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 1 hour.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Mouse Adiponectin/Acrp30 Quantikine ELISA Kit Summary
Background: Adiponectin/Acrp30
Adiponectin (also known as Adipocyte complement-related protein of 30 kDa, Acrp30) is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. Adiponectin associates into 70 kDa noncovalently-linked trimers that are disulfide-linked into medium molecular weight hexamers and then into >300 kDa high molecular weight oligomers.