MANSC Domain Containing Protein 1 (MANSC1) BioAssay™ ELISA Kit (Human)

MANSC Domain Containing Protein 1 (MANSC1) BioAssay™ ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MANSC Domain Containing Protein 1 (MANSC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to MANSC Domain Containing Protein 1 (MANSC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MANSC Domain Containing Protein 1 (MANSC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of MANSC Domain Containing Protein 1 (MANSC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

0.781-50ng/ml

Tests96 Colorimetric Serum, plasma, tissue homogenates and other biological fluids.

<0.27ng/ml

UN2796 PGII

38220000