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Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to carboxy-terminal residues of human MAP2. Antibodies are purified by protein A and peptide affinity chromatography.
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Frozen) | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
Specificity/Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
参考图片
Confocal immunofluorescent analysis of NCI-H522 cells (left, positive) and KATO III cells (right, negative) using MAP2 Antibody (green), DyLight™ 650 Phalloidin #12956 (red), and DAPI #4083 (blue).
Western blot analysis of extracts from PC12 cells, untreated or nocodazole-treated, using MAP2 Antibody.
Western blot analysis of extracts from COS cells, untransfected or MAP2C transfected, using MAP2 Antibody. (Human MAP2C construct is a generous gift from Dr. B. Shafit-Zagardo, Dept. of Pathology, Albert Einstein College of Medicine, Bronx, NY.)
Confocal immunofluorescent analysis of rat cerebellum, hippocampus and spinal cord (as indicated) using MAP2 Antibody (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).