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产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
MAP2
来源纯化
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MAP2 protein.
宿主
Rabbit
商品描述
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Frozen) | 1:200 |
同种型
Rabbit IgG
分子量
75, 82, 280
内毒素水平
人
研究领域
神经科学
应用
反应种属
Mouse,Rat,
预测反应种属
Human
目标/特异性
Specificity/Sensitivity
MAP2 (D5G1) XP Rabbit mAb recognizes endogenous levels of total MAP2 protein. Non-specific labeling of mouse pancreas, colon, small intestine, and liver may be observed by immunofluorescence.
Species Reactivity:
Mouse, Rat
敏感性
Endogenous
背景
背景
Microtubule-associated protein 2 (MAP2) is a neuronal phosphoprotein that regulates the structure and stability of microtubules, neuronal morphogenesis, cytoskeleton dynamics, and organelle trafficking in axons and dendrites (1). Multiple MAP2 isoforms are expressed in neurons, including high molecular weight MAP2A and MAP2B (280 and 270 kDa), and low molecular weight MAP2C and MAP2D (70 and 75 kDa). Phosphorylation of MAP2 modulates its association with the cytoskeleton and is developmentally regulated. GSK-3 and p44/42 MAP kinase phosphorylate MAP2 at Ser136, Thr1620, and Thr1623 (2,3). Phosphorylation at Thr1620/1623 by GSK-3 inhibits MAP2 association with microtubules and microtubule stability (3).
1.Sanchez, C. et al. (2000) Prog. Neurobiol. 61, 133-168.
2.Berling, B. et al. (1994) Eur. J. Cell Biol. 64, 120-130.
3.Sanchez, C. et al. (2000) Eur. J. Cell Biol. 79, 252-260.
研究领域
神经科学
翻译后修饰
unmodified
制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #65911.
数据库链接
Entrez-Gene ID
4133
UniProt ID
P11137
参考图片
Confocal immunofluorescent analysis of frozen rat cerebellum using MAP2 (D5G1) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Western blot analysis of extracts from neonatal and adult mouse brain using MAP2 (D5G1) XP® Rabbit mAb.
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