MAPK Family Antibody Sampler Kit

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MAPK Family Antibody Sampler Kit

品牌： CST

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产品信息

抗原名称

MAPK Family

来源纯化

p44/42 MAP Kinase (137F5) Rabbit mAb is produced by immunizing animals with a synthetic peptide corresponding to residues near the C-terminus of rat p44 MAP kinase. p38 MAPK (D13E1) XP

简单描述

Antibody Sampler Kit for studying p38-alpha MAPK/SAPK/JNK1/ERK1/p38-gamma MAPK/ERK2/p38-beta MAPK in the research area.

研究领域

癌症,细胞生物学,神经科学

应用

目标/特异性

Specificity/Sensitivity

Each antibody in the MAPK Family Antibody Sampler Kit recognizes only its specific target. The antibodies do not cross-react with other MAP kinases.

背景

p44/42 MAPK (Erk1/2), SAPK/JNK, and p38 MAPK function in protein kinase cascades that play a critical role in the regulation of cell growth, differentiation, and control of cellular responses to cytokines and stress. p44/42 MAPK is activated by growth and neurotrophic factors. Activation occurs through phosphorylation of threonine and tyrosine residues (Thr202 and Tyr204 in human Erk1) at the sequence T*EY* by a single upstream MAP kinase kinase (MEK). SAPK/JNK and p38 MAPK are activated by inflammatory cytokines and by a wide variety of cellular stresses. Activation of SAPK/JNK occurs via phosphorylation at Thr183 and Tyr185 by the dual specificity enzyme SEK/MKK4. Both MKK3 and SEK phosphorylate p38 MAPK on tyrosine and threonine at the sequence T*GY* to activate p38 MAP kinase (1-5). 1.Lewis, T. S. et al. (1998) Adv. Cancer Res. 74, 49-139. 2.Garrington, T.P. and Johnson, G.L. (1999) Curr. Opin. Cell. Biol. 11, 211-218. 3.Schaeffer, H.J. and Weber, M.J. (1999) Mol. Cell. Biol. 19, 2435-2444. 4.Whitmarsh, A.J. and Davis, R.J. (1998) Trends Biochem. Sci. 23, 481-485. 5.Cobb, M.H. (1999) Prog. Biophys. Mol. Biol. 71, 479-500.

研究领域

癌症,细胞生物学,神经科学

翻译后修饰

Unmodified

数据库链接

Entrez-Gene ID

1432,5599,5595,6300,5594,5600

UniProt ID

Q16539,P45983,P27361,P53778,P28482,Q15759

参考图片

Western blot analysis of extracts from various cell lines using p38 MAPK (D13E1) XP^® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, showing cytoplasmic and nuclear localization, using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb.

Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells, using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb.

Flow cytometric analysis of Jurkat cells, U0126-treated (blue) or PMA-treated (green), using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb compared to a nonspecific negative control antibody (red).

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb in the presence of control peptide (left) or #1240 p44/42 MAPK (Erk1/2) Blocking Peptide (#4695 Specific) (right).

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using p38 MAPK (D13E1) XP^®Rabbit mAb.

Western blot analysis of extracts from HeLa, NIH/3T3 and C6 cells, using p44/42 MAP Kinase (137F5) Rabbit mAb #4695.

Flow cytometric analysis of HeLa cells using p38 MAPK (D13E1) XP^® Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900 (red).

Western blot analysis of extracts from various cell lines using p38 MAPK (D13E1) XP® Rabbit mAb #8690.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using p38 MAPK (D13E1) XP^®Rabbit mAb.

After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

Western blot analysis of extracts from Hek 293 cells, transfected with 100 nM SignalSilence^® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence^® p44/42 MAPK (Erk1/2) siRNA (+), using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 and α-Tubulin (11H10) Rabbit mAb #2125. The p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb confirms silencing of p44/42 expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of p44/42 MAPK (Erk1/2) siRNA.

Western blot analysis of extracts from 293 and SK-N-MC cells, untreated or UV-treated (40 J/m2), using Phospho-SAPK/JNK Antibody #9251 (upper) or SAPK/JNK Antibody (lower).

Western blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells, using SAPK/JNK (56G8) Rabbit mAb.

Western blot analysis of extracts from HeLa, NIH/3T3, PC12 and COS cells using SAPK/JNK (56G8) Rabbit mAb #9258.

Confocal immunofluorescent analysis of NIH/3T3 cells, treated with either U0126 (MEK1/2 Inhibitor) #9903 (left) or PDGF (right), using p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with UV (100 mJ/cm² with 30 min recovery; right), using p38 MAPK (D13E1) XP^® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using p38 MAPK (D13E1) XP^®Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

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货号：

9926T

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