R&D Systems_mCCL2/JE Aff Pur PAb (100 ug)_优宁维(univ)在线
mCCL2/JE Aff Pur PAb (100 ug)
货号: AF-479-NA 品牌: R&D Systems 反应种属:
Mouse
Mouse
应用:
Western Blot(0.1 µg/mL)
ELISA Capture (Matched Antibody Pair)(0.2-0.8 µg/mL )
ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL )
ELISA Standard ( )
Western Blot(0.1 µg/mL)
ELISA Capture (Matched Antibody Pair)(0.2-0.8 µg/mL )
ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL )
ELISA Standard ( )
规格: 100ug
产品说明书
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价格: ¥5274.00

数量
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产品信息
耦联标记
Unconjugated
纯化方式
Antigen Affinity-purified
目标/特异性
Detects mouse CCL2/JE/MCP-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.01% cross-reactivity with recombinant human (rh) MCP‑1, rhMCP‑2, rhMCP-3, recombinant mouse (rm) MARC, rhMCP-4, rmMCP-5, rmMDC, rmMIP-1 alpha, rmMIP-1 beta, rmMIP-1 gamma,
rmMIP‑2, rmMIP‑3 beta, rmRANTES, rmTARC, and rmTeck is observed.
内毒素水平
<0.10 EU per 1 μg of the antibody by the LAL method.
商品描述
For ELISA the Antibody Pairs information:Capture antibody:AF-479-NA;Detection antibody:BAF479; and protein: 479-JE-010
产品形式
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
应用
实验应用
Western Blot(0.1 µg/mL)
ELISA Capture (Matched Antibody Pair)(0.2-0.8 µg/mL )
ELISA Detection (Matched Antibody Pair)(0.1-0.4 µg/mL )
ELISA Standard ( )
反应种属
Mouse
背景
背景

Mouse CCL2 is a member of the beta (C-C) subfamily of chemokines. The murine CCL2 gene was initially identified as a platelet-derived growth factor-inducible gene in murine fibroblasts. Mouse CCL2 cDNA encodes a 148 amino acid (aa) residue with a putative 23 aa signal peptide that is cleaved to generate the mature protein. Mouse CCL2 shares 82% amino acid sequence identity with rat CCL2. Mouse CCL2 also shares 55% amino acid sequence identity with human MCP-1. Compared to human MCP-1, mouse CCL2 has a 49 aa residue extension at the carboxy-terminus. Mouse CCL2 has full activity on human cells while human MCP-1 has limited activity on mouse cells.

别名
MCAF; MCP-1
制备和贮存
溶解方法
Reconstitute at 0.2 mg/mL in sterile PBS.
文献
文献
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

The Chemokine CCL2 Mediates the Seizure-enhancing Effects of Systemic Inflammation
Authors: C Cerri, S Genovesi, M Allegra, F Pistillo, U Püntener, A Guglielmot, VH Perry, Y Bozzi, M Caleo
J Neurosci, 2016;36(13):3777-88.
Species: Mouse
Sample Type: In Vivo
Application: In vivo
GEC-derived SFRP5 inhibits Wnt5a-induced macrophage chemotaxis and activation.
Authors: Zhao C, Bu X, Wang W, Ma T, Ma H
PLoS ONE, 2014;9(1):e85058.
Species: Human
Sample Type: Whole Cells
Application: Neut
Persistent inflammation subverts thrombospondin-1-induced regulation of retinal angiogenesis and is driven by CCR2 ligation.
Authors: Chen M, Copland DA, Zhao J, Liu J, Forrester JV, Dick AD, Xu H
Am. J. Pathol., 2012;180(1):235-45.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC Frozen
Response patterns of cytokines/chemokines in two murine strains after irradiation.
Authors: Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L
Cytokine, 2012;58(2):169-77.
Species: Mouse
Sample Type: Plasma
Application: Luminex Assay Development
Toll-like receptor 2 contributes to chemokine gene expression and macrophage infiltration in the dorsal root ganglia after peripheral nerve injury.
Authors: Kim D, You B, Lim H, Lee SJ
Mol Pain, 2011;7(0):74.
Species: Mouse
Sample Type: Whole Cells
Application: ICC
Bovine lactoferrin counteracts Toll-like receptor mediated activation signals in antigen presenting cells.
Authors: Puddu P, Latorre D, Carollo M, Catizone A, Ricci G, Valenti P, Gessani S
PLoS ONE, 2011;6(7):e22504.
Species: Mouse
Sample Type: Cell Culture Supernates
Application: ELISA Development
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Authors: Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
J. Clin. Invest., 2010;120(3):907-23.
Species: Mouse
Sample Type: Whole Cells
Application: Flow
Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
J. Immunol., 2009;183(9):5799-806.
Species: Mouse
Sample Type: Cell Culture Supernates
Application: ELISA Development
Stromal MCP-1 in mammary tumors induces tumor-associated macrophage infiltration and contributes to tumor progression.
Authors: Fujimoto H, Sangai T, Ishii G, Ikehara A, Nagashima T, Miyazaki M, Ochiai A
Int. J. Cancer, 2009;125(6):1276-84.
Species: Mouse
Sample Type: In Vivo
Application: In vivo
Reversal of axonal loss and disability in a mouse model of progressive multiple sclerosis.
Authors: Basso AS, Frenkel D, Quintana FJ, Costa-Pinto FA, Petrovic-Stojkovic S, Puckett L, Monsonego A, Bar-Shir A, Engel Y, Gozin M, Weiner HL
J. Clin. Invest., 2008;118(4):1532-43.
Species: Rat
Sample Type: Whole Tissue
Application: IHC Frozen
Expanded-polyglutamine huntingtin protein suppresses the secretion and production of a chemokine (CCL5/RANTES) by astrocytes.
Authors: Chou SY, Weng JY, Lai HL, Liao F, Sun SH, Tu PH, Dickson DW, Chern Y
J. Neurosci., 2008;28(13):3277-90.
Species: Mouse
Sample Type: Whole Cells
Application: ICC
Thrombin induces fibroblast CCL2/JE production and release via coupling of PAR1 to Galphaq and cooperation between ERK1/2 and Rho kinase signaling pathways.
Authors: Deng X, Mercer PF, Scotton CJ, Gilchrist A, Chambers RC
Mol. Biol. Cell, 2008;19(6):2520-33.
Species: Mouse
Sample Type: Whole Cells
Application: ICC
CD14 contributes to pulmonary inflammation and mortality during murine tuberculosis.
Authors: Wieland CW, van der Windt GJ, Wiersinga WJ, Florquin S, van der Poll T
Immunology, 2008;125(2):272-9.
Species: Mouse
Sample Type: Tissue Homogenates
Application: ELISA Development
Megalin contributes to the early injury of proximal tubule cells during nonselective proteinuria.
Authors: Motoyoshi Y, Matsusaka T, Saito A, Pastan I, Willnow TE, Mizutani S, Ichikawa I
Kidney Int., 2008;74(10):1262-9.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC Paraffin-embedded
Role of the chemokine decoy receptor D6 in balancing inflammation, immune activation, and antimicrobial resistance in Mycobacterium tuberculosis infection.
Authors: Di Liberto D, Locati M, Caccamo N, Vecchi A, Meraviglia S, Salerno A, Sireci G, Nebuloni M, Caceres N, Cardona PJ, Dieli F, Mantovani A
J. Exp. Med., 2008;205(9):2075-84.
Species: Mouse
Sample Type: In Vivo
Application: In vivo
Vascular and inflammatory stresses mediate atherosclerosis via RAGE and its ligands in apoE-/- mice.
Authors: Harja E, Bu DX, Hudson BI, Chang JS, Shen X, Hallam K, Kalea AZ, Lu Y, Rosario RH, Oruganti S, Nikolla Z, Belov D, Lalla E, Ramasamy R, Yan SF, Schmidt AM
J. Clin. Invest., 2008;118(1):183-94.
Species: Mouse
Sample Type: Cell Lysates
Application: WB
TWEAK/Fn14 interactions are instrumental in the pathogenesis of nephritis in the chronic graft-versus-host model of systemic lupus erythematosus.
Authors: Zhao Z, Burkly LC, Campbell S, Schwartz N, Molano A, Choudhury A, Eisenberg RA, Michaelson JS, Putterman C
J. Immunol., 2007;179(11):7949-58.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC Paraffin-embedded
Chemical interactions between fibrosarcoma cancer cells and sensory neurons contribute to cancer pain.
Authors: Khasabova IA, Stucky CL, Harding-Rose C, Eikmeier L, Beitz AJ, Coicou LG, Hanson AE, Simone DA, Seybold VS
J. Neurosci., 2007;27(38):10289-98.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC Frozen
The chemokine decoy receptor M3 blocks CC chemokine ligand 2 and CXC chemokine ligand 13 function in vivo.
Authors: Martin AP, Canasto-Chibuque C, Shang L, Rollins BJ, Lira SA
J. Immunol., 2006;177(10):7296-302.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC Paraffin-embedded
Adenoviral-mediated overexpression of SOCS3 enhances IgG immune complex-induced acute lung injury.
Authors: Gao H, Hoesel LM, Guo RF, Rancilio NJ, Sarma JV, Ward PA
J. Immunol., 2006;177(1):612-20.
Species: Human
Sample Type: BALF
Application: ELISA Development
An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
J. Exp. Med., 2006;203(12):2763-77.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC Frozen
CCL2 regulates angiogenesis via activation of Ets-1 transcription factor.
Authors: Stamatovic SM, Keep RF, Mostarica-Stojkovic M, Andjelkovic AV
J. Immunol., 2006;177(4):2651-61.
Species: Mouse
Sample Type: Whole Cells
Application: Neut
Circulating cytokine/inhibitor profiles reshape the understanding of the SIRS/CARS continuum in sepsis and predict mortality.
Authors: Osuchowski MF, Welch K, Siddiqui J, Remick DG
J. Immunol., 2006;177(3):1967-74.
Species: Mouse
Sample Type: Plasma
Application: ELISA Development
Antibody-bound beta-amyloid precursor protein stimulates the production of tumor necrosis factor-alpha and monocyte chemoattractant protein-1 by cortical neurons.
Authors: Gonzalez de Aguilar JL, Dupuis L, Larmet Y
Neurobiol. Dis., 2005;19(1):129-41.
Species: Mouse
Sample Type: Cell Lysates
Application: WB
Sequential ELISA to profile multiple cytokines from small volumes.
Authors: Osuchowski MF, Siddiqui J, Copeland S, Remick DG
J. Immunol. Methods, 2005;302(1):172-81.
Species: Mouse
Sample Type: Plasma
Application: ELISA Development
Involvement of monocyte chemoattractant protein-1, macrophage inflammatory protein-1alpha and interleukin-1beta in Wallerian degeneration.
Authors: Perrin FE, Lacroix S, Aviles-Trigueros M, David S
Brain, 2005;128(0):854-66.
Species: Mouse
Sample Type: In Vivo
Application: In vivo
Selective macrophage suppression during sepsis.
Authors: Ellaban E, Bolgos G, Remick D
Cell. Immunol., 2004;231(1):103-11.
Species: Mouse
Sample Type: Cell Culture Supernates
Application: ELISA Development
17beta-estradiol (E2) modulates cytokine and chemokine expression in human monocyte-derived dendritic cells.
Authors: Bengtsson AK, Ryan EJ, Giordano D, Magaletti DM, Clark EA
Blood, 2004;104(5):1404-10.
Species: Human
Sample Type: Cell Culture Supernates
Application: ELISA Development
A critical role for CCR2/MCP-1 interactions in the development of idiopathic pneumonia syndrome after allogeneic bone marrow transplantation.
Authors: Hildebrandt GC, Duffner UA, Olkiewicz KM, Corrion LA, Willmarth NE, Williams DL, Clouthier SG, Hogaboam CM, Reddy PR, Moore BB, Kuziel WA, Liu C, Yanik G, Cooke KR
Blood, 2004;103(6):2417-26.
Species: Mouse
Sample Type: Whole Tissue
Application: IHC
Possible co-regulation of genes associated with enhanced progression of mammary adenocarcinomas.
Authors: Neumark E, Cohn MA, Lukanidin E, Witz IP, Ben-Baruch A
Immunol. Lett., 2002;82(1):111-21.
Species: Mouse
Sample Type: Cell Culture Supernates
Application: DB
The chemokine KC, but not monocyte chemoattractant protein-1, triggers monocyte arrest on early atherosclerotic endothelium.
Authors: Huo Y, Weber C, Forlow SB, Sperandio M, Thatte J, Mack M, Jung S, Littman DR, Ley K
J. Clin. Invest., 2001;108(9):1307-14.
Species: Mouse
Sample Type: Whole Cells
Application: Neut
Role of CC chemokines (macrophage inflammatory protein-1 beta, monocyte chemoattractant protein-1, RANTES) in acute lung injury in rats.
Authors: Bless NM, Huber-Lang M, Guo RF, Warner RL, Schmal H, Czermak BJ, Shanley TP, Crouch LD, Lentsch AB, Sarma V, Mulligan MS, Friedl HP, Ward PA
J. Immunol., 2000;164(5):2650-9.
Species: Rat
Sample Type: BALF
Application: ELISA Development
Pulmonary Epithelial Cell-Derived Cytokine TGF-beta1 Is a Critical Cofactor for Enhanced Innate Lymphoid Cell Function.
Authors: Denney L, Byrne A, Shea T, Buckley J, Pease J, Herledan G, Walker S, Gregory L, Lloyd C
Immunity, 0;43(5):945-58.
Species: Mouse
Sample Type: Tissue HomogenatesLung
Application: ELISA Capture

参考图片

Chemotaxis Induced by CCL2/JE and Neutralization by Mouse CCL2/JE Antibody. Recombinant Mouse CCL2/JE (Catalog # 479-JE) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CCL2/JE (0.04 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL2/JE Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-479-NA). The ND50 is typically 0.5-2.5 µg/mL.

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