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mCXCL16 MAb (Cl 1424 (100 ug)

mCXCL16 MAb (Cl 1424 (100 ug)

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品牌: R&D Systems
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来源宿主:
Rat IgG2a
实验应用:
Western Blot, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard , Neutralization
产品介绍
产品介绍
产品信息
荧光素标记
纯化方式
Protein A or G purified from hybridoma culture supernatant
抗原名称
chemokine (C-X-C motif) ligand 16, CXC chemokine ligand 16, CXCL16, CXCLG16, Scavenger receptor for
宿主
Rat IgG2a
免疫原
E. coli-derived recombinant mouse CXCL16
Asn27-Pro114
Accession # Q8BSU2
克隆号
142417

Scientific Data

Neutralization Chemotaxis Induced by CXCL16 and Neutralization by Mouse CXCL16 Antibody.View Larger

Chemotaxis Induced by CXCL16 and Neutralization by Mouse CXCL16 Antibody. Recombinant Mouse CXCL16 Chemokine Domain (Catalog # 503-CX) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CXCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL16 Chemokine Domain (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL16 Monoclonal Antibody (Catalog # MAB503). The ND50 is typically 0.15-0.6 µg/mL.

Mouse CXCL16 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CXCL16 in ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CXCL1, 2, 3, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, recombinant mouse CXCL1, 2, 6, 9, 10, 11, 12, 13, 14, 15, recombinant rat CXCL1, 2, 3, 5, or recombinant porcine CXCL8 is observed.
Source
Monoclonal Rat IgG2A Clone # 142417
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse CXCL16
Asn27-Pro114
Accession # Q8BSU2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CXCL16 Chemokine Domain (Catalog # 503-CX) under non-reducing conditions only

Mouse CXCL16 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse CXCL16 Biotinylated Antibody (Catalog # BAF503)

Standard: Recombinant Mouse CXCL16 Protein (Catalog # 503-CX)

Neutralization
Measured by its ability to neutralize CXCL16-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CXCR6. Matloubian, M. et al. (2000) Nature Immunol. 1:298. The Neutralization Dose (ND50) is typically 0.15-0.6 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse CXCL16 Chemokine Domain.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

应用
实验应用
Western Blot, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard , Neutralization
背景
别名
chemokine (C-X-C motif) ligand 16,CXC chemokine ligand 16,CXCL16,CXCLG16,Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein,SCYB16,Small-inducible cytokine B16,SRPSOXC-X-C motif chemokine 16,SR-PSOXTransmembrane chemokine CXCL16
背景

Background: CXCL16

Mouse CXCL16 (CXC chemokine 16) is a non-ELR motif-containing CXC chemokine with a transmembrane domain. CX3CL1/Fractalkine andCXCL16 are the only two transmembrane chemokines within the superfamily. Mouse CXCL16 cDNA encodes a 246 amino acid (aa) precursor protein with a putative 26 aa signal peptide, an 88 aa chemokine domain, an 87 aa mucin-like spacer region, a 22 aa transmembrane domain, and a 23 aa cytoplasmic tail. Mouse and human CXCL16 share 49% aa sequence identity. Mouse CXCL16 is produced by dendritic cells in lymphoid organ T cell zones and bycells in the splenic red pulp both as membrane-bound and soluble forms. Based on northern blot analysis, CXCL16 is also expressed in some non-lymphoid tissues such as lung, small intestine and kidney. The receptor for CXCL16 has been identified as CXCR6 (previously called Bonzo, STRL33 or TYMSTR), which is also a coreceptor for HIV entry. CXCR6 is expressed on naive CD8 cells, natural killer T cells and activated CD8 and CD4 T cells.

Entrez Gene IDs
58191 (Human); 66102 (Mouse)
Alternate Names
chemokine (C-X-C motif) ligand 16; CXC chemokine ligand 16; CXCL16; CXCLG16; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SCYB16; Small-inducible cytokine B16; SRPSOXC-X-C motif chemokine 16; SR-PSOXTransmembrane chemokine CXCL16
制备和贮存
溶解方法
Reconstitute at 0.5 mg/mL in sterile PBS.
保存方式

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

参考图片

Chemotaxis Induced by CXCL16 and Neutralization by Mouse CXCL16 Antibody. Recombinant Mouse CXCL16 Chemokine Domain (Catalog # 503-CX) chemoattracts the BaF3 mouse pro‑B cell line transfected with mouse CXCR6 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL16 Chemokine Domain (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse CXCL16 Monoclonal Antibody (Catalog # MAB503). The ND50 is typically 0.15‑0.6 µg/mL.

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货号:
MAB503-100
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