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mGranzyme B Biot Aff (50 ug)
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mGranzyme B Biot Aff (50 ug)

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品牌: R&D Systems
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来源宿主:
Goat IgG
实验应用:
Western Blot, Immunohistochemistry, Immunocytochemistry, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard
产品介绍
产品介绍
产品信息
荧光素标记
纯化方式
Antigen Affinity-purified
抗原名称
C11, CCPI, CGL1, CGL-1, CSPB, CSP-B, CTLA1, CTLA-1, CTSGL1, Fragmentin-2, Granzyme B, Granzyme-2, Gr
宿主
Goat IgG
免疫原
Mouse myeloma cell line NS0-derived recombinant mouse Granzyme B
Lys17-Ser247
Accession # P04187

Scientific Data

Immunohistochemistry Granzyme B antibody in Mouse Spleen by Immunohistochemistry (IHC-Fr).View Larger

Granzyme B in Mouse Spleen. Granzyme B was detected in immersion fixed frozen sections of mouse spleen using Goat Anti-Mouse Granzyme B Biotinylated Antigen Affinity-purified Poly-clonal Antibody (Catalog # BAF1865) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Immunocytochemistry View Larger

Granzyme B in Mouse Splenocytes. Granzyme B was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse Granzyme B Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1865) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Mouse Granzyme B Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Granzyme B in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human (rh) Granzyme A, rhGranzyme B, recombinant mouse (rm) Granzyme D, rmGranzyme G, and rhGranzyme H is observed.
Source
Polyclonal Goat IgG
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Granzyme B (Catalog # 1865-SE)
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
Immersion fixed mouse splenocytes

Mouse Granzyme B Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Mouse Granzyme B Antibody (Catalog # AF1865)

Standard: Recombinant Mouse Granzyme B Protein, CF (Catalog # 1865-SE)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

应用
实验应用
Western Blot, Immunohistochemistry, Immunocytochemistry, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard
背景
别名
C11,CCPI,CGL1,CGL-1,CSPB,CSP-B,CTLA1,CTLA-1,CTSGL1,Fragmentin-2,Granzyme B,Granzyme-2,GranzymeB,GRB,GrzB,GZMB,HLP,SECT
背景

Background: Granzyme B

Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various caspases and cleaves proteins such as aggrecan (3). Mouse Granzyme B is synthesized as a precursor (247 residues) with a signal and a pro peptide (residues 1-20) and a mature chain (residues 21‑247) (4, 5). The recombinant mouse Granzyme B consisting of residues 17 to 247 was expressed and purified. After activation with cathepsin C, it cleaves a thioester substrate.

References
  1. Kam, C.-M. et al. (2000) Biochim. Biophys. Acta 1477:307.
  2. Smyth, M.J. et al. (1996) J. Leukoc. Biol. 60:555.
  3. Froelich, C.J. (2004) in Handbook of Proteolytic Enzymes, Barrett, A.J. et al. eds. p. 1549.
  4. Lobe, C.G. et al. (1986) Science 232:858.
  5. Brunet, J.F. et al. (1986) Nature 322:268.
Entrez Gene IDs
3002 (Human); 14939 (Mouse)
Alternate Names
C11; CCPI; CGL1; CGL-1; CSPB; CSP-B; CTLA1; CTLA-1; CTSGL1; Fragmentin-2; Granzyme B; Granzyme-2; GranzymeB; GRB; GrzB; GZMB; HLP; SECT
制备和贮存
溶解方法
Reconstitute at 0.2 mg/mL in sterile PBS.
保存方式

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

参考图片

Granzyme B in Mouse Spleen. Granzyme B was detected in immersion fixed frozen sections of mouse spleen using Goat Anti-Mouse Granzyme B Biotinylated Antigen Affinity-purified Poly­clonal Antibody (Catalog # BAF1865) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counter­stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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货号:
BAF1865
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询价
50ug
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