R&D Systems_mGranzyme B Biot Aff (50 ug)

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mGranzyme B Biot Aff (50 ug)

货号： BAF1865 品牌： R&D Systems 反应种属：

Mouse

应用：

Western Blot, Immunohistochemistry, Immunocytochemistry, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard

规格： 50ug

产品说明书

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价格: ¥5919.00

数量

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产品信息

耦联标记

Biotin

纯化方式

Antigen Affinity-purified

目标/特异性

Detects mouse Granzyme B in ELISAs and Western blots. In sandwich immunoassays, less than 1% cross-reactivity with recombinant human (rh) Granzyme A, rhGranzyme B, recombinant mouse (rm) Granzyme D, rmGranzyme G, and rhGranzyme H is observed.

商品描述

For ELISA the Antibody Pairs information：Capture antibody：AF1865；Detection antibody：BAF1865; and protein: 1865-SE-010

产品形式

Reconstitute at 0.2 mg/mL in sterile PBS.

应用

实验应用

Western Blot, Immunohistochemistry, Immunocytochemistry, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard

反应种属

Mouse

背景

Granzyme B is a member of the granzyme family of the serine proteases found specifically in the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells (1, 2). Granzyme B plays an essential role in granule-mediated apoptosis and may have additional roles in rheumatoid arthritis and in bacterial and viral infections (3). It activates various caspases and cleaves proteins such as aggrecan (3). Mouse Granzyme B is synthesized as a precursor (247 residues) with a signal and a pro peptide (residues 1-20) and a mature chain (residues 21‑247) (4, 5). The recombinant mouse Granzyme B consisting of residues 17 to 247 was expressed and purified. After activation with cathepsin C, it cleaves a thioester substrate.

别名

CSPB; CTLA-1; CTSGL1; Fragmentin-2; Granzyme-2; GRB; GrzB; GZMB; HLP; SECT

制备和贮存

溶解方法

Reconstitute at 0.2 mg/mL in sterile PBS.

文献

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

Hematopoietic stem cell function and survival depend on c-Myc and N-Myc activity.
Authors: Laurenti E, Varnum-Finney B, Wilson A, Ferrero I, Blanco-Bose WE, Ehninger A, Knoepfler PS, Cheng PF, MacDonald HR, Eisenman RN, Bernstein ID, Trumpp A
Cell Stem Cell, 2008;3(6):611-24.
Species: Mouse
Sample Type: Whole Cells
Application: ICC

参考图片

Granzyme B in Mouse Spleen. Granzyme B was detected in immersion fixed frozen sections of mouse spleen using Goat Anti-Mouse Granzyme B Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1865) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

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