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MHC Class I (EMR8-5) Mouse mAb

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MHC Class I (EMR8-5) Mouse mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

来源纯化

Monoclonal antibody is produced by immunizing animals with human HLA-A*2402 extracellular domain recombinant protein.

宿主

Mouse

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunohistochemistry (Paraffin)	1:100 - 1:400
Flow Cytometry (Fixed/Permeabilized)	1:200 - 1:800

同种型

Mouse IgG1 kappa

分子量

30-50

应用

目标/特异性

Specificity/Sensitivity

MHC Class I (EMR8-5) Mouse mAb detects HLA-A, HLA-B, HLA-C, and HLA-E. This antibody does not cross-react with HLA-G.

Species Reactivity:

Human

敏感性

Endogenous

背景

The human leukocyte antigen (HLA) system is a gene complex encoding the major histocompatibility complex (MHC) proteins in humans. These cell surface proteins are responsible for the regulation of antigen-specific immunity in humans. HLA genes are highly polymorphic, allowing them to fine-tune the adaptive immune response. HLAs corresponding to MHC class I (HLA-A, B, and C) present small peptide antigens from inside the cell, approximately 8 to 10 amino acids in length, to CD8+ T lymphocytes in order to activate a cytotoxic T cell response. HLAs corresponding to MHC class II (HLA-DP, DM, DO, DQ, and DR) present antigens from outside of the cell, approximately 15 to 24 residues in length, to CD4+ T helper cells, which in turn secrete cytokines and stimulate B cells to produce antibodies to that specific antigen. HLAs corresponding to MHC class III encode components of the complement system (1,2). 1.Marsh, S.G. et al. (2005) Hum Immunol 66, 571-636. 2.Shiina, T. et al. (2004) Tissue Antigens 64, 631-49.

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #28149.

数据库链接

Entrez-Gene ID

3105,3107,3106

UniProt ID

P04439,P10321,P01889_VAR_C349S

参考图片

Flow cytometric analysis of K-562 cells (blue, low-expressing) and HL-60 cells (green, high-expressing) using MHC Class I (EMR8-5) Mouse mAb (solid lines) or concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (dashed lines). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.

Western blot analysis of extracts from various cell lines using MHC Class I (EMR8-5) Mouse mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). Low expression of MHC Class I protein in K-562 cells is consistent with the predicted expression pattern.

Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma using MHC Class I (EMR8-5) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded human papillary carcinoma of the thyroid using MHC Class I (EMR8-5) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded human gastric carcinoma using MHC Class I (EMR8-5) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded human B-cell non-Hodgkin lymphoma using MHC Class I (EMR8-5) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded human large cell neuroendocrine lung carcinoma using MHC Class I (EMR8-5) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded human colon adenocarcinoma using MHC Class I (EMR8-5) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the cervix using MHC Class I (EMR8-5) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using MHC Class I (EMR8-5) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded normal human spleen using MHC Class I (EMR8-5) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded normal human small intestine using MHC Class I (EMR8-5) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded normal human pancreas using MHC Class I (EMR8-5) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded normal human kidney using MHC Class I (EMR8-5) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded normal human heart using MHC Class I (EMR8-5) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded normal human colon using MHC Class I (EMR8-5) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma using MHC Class I (EMR8-5) Mouse mAb (left) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (right).

Immunohistochemical analysis of paraffin-embedded HL-60 cell pellet (left, high-expressing) or K-562 cell pellet (right, low-expressing) using MHC Class I (EMR8-5) Mouse mAb.

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货号：

88274T

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询价

供应商现货

20μl

100ul

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当前规格1件起购

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