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mIP-10 MAb (Cl 13401 (25 ug)

mIP-10 MAb (Cl 13401 (25 ug)

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品牌: R&D Systems
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来源宿主:
Rat IgG2a
实验应用:
Western Blot, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard , Neutralization
产品介绍
产品介绍
产品信息
荧光素标记
纯化方式
Protein A or G purified from hybridoma culture supernatant
抗原名称
C7, chemokine (C-X-C motif) ligand 10, CRG2, CRG-2, CXCL10, gIP-10, IFI10, INP10, IP-10, mob-1, SCYB
宿主
Rat IgG2a
免疫原
E. coli-derived recombinant mouse CXCL10/IP-10/CRG-2
Ile22-Pro98
Accession # P17515
克隆号
134013

Scientific Data

Neutralization Chemotaxis Induced by CXCL10/CRG‑2 and Neutralization by Mouse CXCL10/CRG‑2 Antibody.View Larger

Chemotaxis Induced by CXCL10/CRG‑2 and Neutralization by Mouse CXCL10/CRG‑2 Antibody. Recombinant Mouse CXCL10/CRG‑2 (Catalog # 466-CR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL10/CRG‑2 (0.5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CXCL10/CRG‑2 Monoclonal Antibody (Catalog # MAB466). The ND50 is typically 10-40 µg/mL.

Mouse CXCL10/IP-10/CRG-2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse CXCL10/IP-10/CRG-2 in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) CXCL1, 2, 3, 5, 6, 7, 9, 10, 11, 12/SDF-1 alpha, 12/SDF-1 beta, 13, rmCXCL1, 2, 6, 9, 11, 12/SDF-1 alpha, 13, rpCXCL8, rrCXCL1, 3/CINC-2 alpha, 3/CINC-2 beta
Source
Monoclonal Rat IgG2A Clone # 134013
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse CXCL10/IP-10/CRG-2
Ile22-Pro98
Accession # P17515
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse CXCL10/IP‑10/CRG‑2 (Catalog # 466-CR)

Mouse CXCL10/IP-10/CRG-2 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse CXCL10/IP‑10/CRG‑2 Biotinylated Antibody (Catalog # BAF466)

Standard: Recombinant Mouse CXCL10/IP-10/CRG-2 Protein (Catalog # 466-CR)

Neutralization
Measured by its ability to neutralize CXCL10/IP‑10/CRG‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CXCR3. The Neutralization Dose (ND50) is typically 10-40 µg/mL in the presence of 0.5 µg/mL Recombinant Mouse CXCL10/IP‑10/CRG‑2.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

应用
实验应用
Western Blot, ELISA Capture (Matched Antibody Pair), ELISA Detection (Matched Antibody Pair), ELISA Standard , Neutralization
背景
别名
C7,chemokine (C-X-C motif) ligand 10,CRG2,CRG-2,CXCL10,gIP-10,IFI10,INP10,IP-10,mob-1,SCYB10
背景

Background: CXCL10/IP-10/CRG-2

The gene for CRG-2, a mouse homolog of human IP-10, was originally identified as an immediate early gene induced in response to macrophage activation. It has since been shown that CRG-2 mRNA is induced by alpha / beta / gamma -interferons and by lipopolysaccharide in macrophages, astrocytes and microglia. Human IP-10 was also shown to be expressed in activated T-lymphocytes, splenocytes, keratinocytes, osteoblasts, astrocytes, and smooth muscle cells. Mouse CRG-2 cDNA encodes a 98 amino acid (aa) residue precursor protein with a 21 aa residue signal peptide that is cleaved to form the 77 aa residue secreted mature protein. Mature CRG-2 shares approximately 67% amino acid sequence identity with human IP-10. The amino acid sequence of CRG-2 identified the protein as a member of the chemokine alpha subfamily that lacks the ELR domain. CRG-2 has been shown to be a chemoattractant for activated T-lymphocytes. Recently, human IP-10 has also been reported to be a potent inhibitor of angiogenesis and to display a potent thymus-dependent anti-tumor effect. A chemokine receptor specific for IP-10 and MIG (CXCR3) has been cloned and shown to be highly expressed in IL-2-activated T-lymphocytes.

References
  1. Loetscher, M. et al. (1996) J. Exp. Med. 184:963.
  2. Vanguri, P. (1996) J. Neuroimmunol. 56:35.
  3. Sgadari, C. et al. (1996) Blood, 87:3877.
Entrez Gene IDs
3627 (Human); 15945 (Mouse)
Alternate Names
C7; chemokine (C-X-C motif) ligand 10; CRG2; CRG-2; CXCL10; gIP-10; IFI10; INP10; IP-10; mob-1; SCYB10
制备和贮存
溶解方法
Reconstitute at 0.5 mg/mL in sterile PBS.
保存方式

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

参考图片

Chemotaxis Induced by CXCL10/CRG‑2 and Neutralization by Mouse CXCL10/CRG‑2 Antibody. Recombinant Mouse CXCL10/CRG‑2 (Catalog # 466-CR) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Mouse CXCL10/CRG‑2 (0.5 µg/mL) is neutralized (green line) by increasing concentrations of Mouse CXCL10/CRG‑2 Monoclonal Antibody (Catalog # MAB466). The ND50 is typically 10-40 µg/mL.

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货号:
MAB466-SP
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