全部商品分类
下载产品说明书 下载COA 下载SDS
用小程序，查商品更便捷
收藏
对比
咨询MitoTracker® Red CMXRos is well retained after fixation allowing for further sample processing and immunostaining. Excitation: 579 nm, Emission: 599 nm, Molecular Weight: 531.52
Product Usage Information
Each vial of MitoTracker® Red CMXRos contains 50 μg lyophilized solid. To make a 1 mM stock solution, reconstitute the solid in 94.1 μl high quality DMSO. While the optimal staining concentration and incubation times can vary by application, typical results are obtained by diluting the stock solution directly into growth media at a concentration between 50-200 nM and incubation between 15-45 min at 37ºC. After incubation, fix the cells in ice-cold methanol for 15 min at -20ºC then rinse 3 times with PBS for 5 min. Proceed with immunostaining.
Store lyophilized solid at -20°C dessicated and protected from light. In lyophilized form, this reagent is stable for 12 months. Once reconstituted in DMSO, the solution can be stored at -20°C, protected from light and must be used within 2 weeks. Avoid freeze-thaw cycles.
参考图片
Flow cytometric analysis of live Jurkat cells, treated with CCCP (80 μM, 15 min; blue) or untreated (green) labeled with MitoTracker® Red CMXRos (solid lines), compared to unlabeled Jurkat cells (dashed line).
Confocal immunofluorescent analysis of HeLa cells using MitoTracker® Red CMXRos (red) and Lamin A/C (4C11) Mouse mAb (Alexa Fluor® 488 Conjugate) #8617 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).