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MMP-2 (D4M2N) Rabbit mAb

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MMP-2 (D4M2N) Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

来源纯化

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human MMP-2 protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100
Immunohistochemistry (Paraffin)	1:75 - 1:300
Immunofluorescence (Immunocytochemistry)	1:200 - 1:800

同种型

Rabbit IgG

分子量

64,72

应用

目标/特异性

Specificity/Sensitivity

MMP-2 (D4M2N) Rabbit mAb recognizes endogenous levels of total MMP-2 protein.

Species Reactivity:

Human

敏感性

Endogenous

背景

The matrix metalloproteinases (MMPs) are a family of proteases that target many extracellular proteins including other proteases, growth factors, cell surface receptors, and adhesion molecules (1). Among the family members, MMP-2, MMP-3, MMP-7, and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis, and apoptosis (2-4). Research studies have shown that MMP activity correlates with cancer development (2). One mechanism of MMP regulation is transcriptional (5). Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full-length protein (5). 1.McCawley, L.J. and Matrisian, L.M. (2001) Curr Opin Cell Biol 13, 534-40. 2.Coussens, L.M. et al. (2002) Science 295, 2387-92. 3.Sternlicht, M.D. et al. (1999) Cell 98, 137-46. 4.Vu, T.H. et al. (1998) Cell 93, 411-22. 5.Nagase, H. et al. (1990) Biochemistry 29, 5783-9.

研究领域

癌症,细胞生物学,纤维化,神经科学,

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #35814.

数据库链接

Entrez-Gene ID

4313

UniProt ID

P08253

参考图片

Confocal immunofluorescent analysis of U-87MG cells (left, positive) and HCT-15 cells (right, negative) using MMP-2 (D4M2N) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western blot analysis of extracts from various cell lines using MMP-2 (D4M2N) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Immunoprecipitation of MMP-2 protein from U-118MG cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MMP-2 (D4M2N) Rabbit mAb. Western blot analysis was performed using MMP-2 (D4M2N) Rabbit mAb as primary antibody. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as secondary antibody.

Immunohistochemical analysis of paraffin-embedded human bladder adenocarcinoma using MMP-2 (D4M2N) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded U-87 MG cell pellet (left, positive) or HCT-15 cell pellet (right, negative) using MMP-2 (D4M2N) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human cervical carcinoma using MMP-2 (D4M2N) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma using MMP-2 (D4M2N) Rabbit mAb.

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货号：

40994S

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