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MMP-9 (D6O3H) XP® Rabbit mAb

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MMP-9 (D6O3H) XP® Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

MMP-9

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe542 of human MMP-9 protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunohistochemistry (Paraffin)	1:150 - 1:600
Flow Cytometry (Fixed/Permeabilized)	1:200 - 1:800

同种型

Rabbit IgG

分子量

84, 92

研究领域

癌症,细胞生物学,纤维化,神经科学,

应用

反应种属

Human

目标/特异性

Specificity/Sensitivity

MMP-9 (D6O3H) XP Rabbit mAb recognizes the full-length, proenzyme (92 kDa) and the cleaved, active enzyme (84 kDa) of MMP-9.

Species Reactivity:

Human

敏感性

Endogenous

背景

The matrix metalloproteinases (MMPs) are a family of proteases that target many extracellular proteins including other proteases, growth factors, cell surface receptors, and adhesion molecules (1). Among the family members, MMP-2, MMP-3, MMP-7, and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis, and apoptosis (2-4). Research studies have shown that MMP activity correlates with cancer development (2). One mechanism of MMP regulation is transcriptional (5). Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full-length protein (5). 1.McCawley, L.J. and Matrisian, L.M. (2001) Curr Opin Cell Biol 13, 534-40. 2.Coussens, L.M. et al. (2002) Science 295, 2387-92. 3.Sternlicht, M.D. et al. (1999) Cell 98, 137-46. 4.Vu, T.H. et al. (1998) Cell 93, 411-22. 5.Nagase, H. et al. (1990) Biochemistry 29, 5783-9.

研究领域

癌症,细胞生物学,纤维化,神经科学,

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier-free (BSA and azide free) version of this product see product #15749.

数据库链接

Entrez-Gene ID

4318

UniProt ID

P14780

参考图片

Flow cytometric analysis of U-2 OS cells, untreated (blue) or treated with TPA (12-O-Tetradecanoylphorbol-13-Acetate) #4174 (200 nM, 24 hr; green) using MMP-9 (D6O3H) XP® Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed lines). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

Western blot analysis of concentrated, serum-free cultured medium from U-2 OS cells, untreated (-) or treated with TPA #4174 (200 nM, 48 hr; +), using MMP-9 (D6O3H) XP® Rabbit mAb.

Western blot analysis of extracts and concentrated culture medium from U-2 OS cells, untreated (-) or treated with TPA #4174 (200 nM, 48 hr; +) using MMP-9 (D6O3H) XP® Rabbit mAb. MMP-9 is induced by TPA treatment as expected.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using MMP-9 (D6O3H) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using MMP-9 (D6O3H) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded U-2 OS cell pellets, untreated (left) or treated with TPA #4174 (right), using MMP-9 (D6O3H) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using MMP-9 (D6O3H) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

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货号：

13667T

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