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Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys115 of human MMP-9. Antibodies are purified by protein A and peptide affinity chromatography.
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Specificity/Sensitivity
Species Reactivity:
Human
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
参考图片
Immunoprecipitation of MMP-9 protein from U-2 OS cell extracts. Cells were treated with TPA #4174 (200 nM, 48 hr; +). Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is MMP-9 Antibody. Western blot analysis was performed using MMP-9 (D6O3H) XP® Rabbit mAb #13667.
Western blot analysis of partially purified, concentrated, serum-free medium containing human MMP-9, using MMP-9 Antibody.
Western blot analysis of extracts and concentrated culture medium from U-2 OS cells, untreated (-) or treated with TPA #4174 (200 nM, 48 hr; +) using MMP-9 Antibody. MMP-9 is induced by TPA treatment as expected.