Monkey Interleukin 5 ELISA Kit

interleukin-5

Principle of the Assay: IL-5 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for IL-5. Standards or samples are then added to the microtiter plate wells and IL-5 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of IL-5 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for IL-5 are added to each well to "sandwich" the IL-5 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain IL-5 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL-5 concentration in each sample is interpolated from this standard curve.

微量滴定板（涂覆抗猴白细胞介素5抗体96孔板），酶结合物（辣根过氧化物酶标记的链霉亲和素），标准品A/B/C/D/E/F（重组抗猴白细胞介素5蛋白），底物溶液A（四甲基联苯胺溶液），底物溶液B（20%氢氧化钠溶液），终止液（20%氢氧化钠溶液），100×清洗液 （0.05%吐温20、99.95%水），平衡溶液（对硝基苯酚磷酸盐溶液）

Monkey

50-1000pg/mL

Sandwich

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

1.0pg/mL

24497

Q08125 [Other Products]