Monkey Leptin ELISA Kit

leptin

Principle of the Assay: Leptin ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Leptin antibody and an Leptin-HRP conjugate. The assay sample and buffer are incubated together with Leptin-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Leptin concentration since Leptin from samples and Leptin-HRP conjugate compete for the anti-Leptin antibody binding site. Since the number of sites is limited, as more sites are occupied by Leptin from the sample, fewer sites are left to bind Leptin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Leptin concentration in each sample is interpolated from this standard curve.

微量滴定板（涂覆抗猴瘦蛋白抗体96孔板），酶结合物（辣根过氧化物酶标记的链霉亲和素），标准品A/B/C/D/E/F（重组抗猴瘦蛋白），底物溶液A（四甲基联苯胺溶液），底物溶液B（20%氢氧化钠溶液），终止液（20%氢氧化钠溶液），100×清洗液（0.05%吐温20、99.95%水），平衡溶液（对硝基苯酚磷酸盐溶液），操作说明书

Monkey

250-5000pg/ML

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate

1.0pg/mL

734226