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Fig.1. SDS-PAGE analysis of MMAF-1-88H4
Lane M: Precision Plus Protein™ Dual Color Standards (Bio-Rad) (Sample loaded: 5 μl)
Lane 1: Purified MMAF-1-88H4 under reducing condition (Sample loaded: 2 μg)
Lane 2: Purified MMAF-1-88H4 under non-reducing condition (Sample loaded: 2 μg)
•\tRecombinant protein was visualized by Coomassie Brilliant Blue R250 staining.
Store at -80ºC. Avoid multiple freeze-thaw cycles.
参考图片
Fig.1. SDS-PAGE analysis of MMAF-1-88H4
Lane M: Precision Plus Protein™ Dual Color Standards (Bio-Rad) (Sample loaded: 5 μl)
Lane 1: Purified MMAF-1-88H4 under reducing condition (Sample loaded: 2 μg)
Lane 2: Purified MMAF-1-88H4 under non-reducing condition (Sample loaded: 2 μg)
• Recombinant protein was visualized by Coomassie Brilliant Blue R250 staining.
Fig. 2. SEC-HPLC analysis of MMAF-1-88H4
Running buffer: 2*PBS, pH 7.4
Column: Superdex 200 Increase 5/150 GL(GE, Code Number: 28-9909-45)
Fig.4. Sandwich ELISA analysis of MMAF-1-88H4 (CP2023013101)
Coating Her2 ECD protein: 2ug/ml
Add MMAF/MMAE-ADC: from 10ug/ml, 10-fold diluent, 8 points including 0
Add MMAF antibody 88H4 (mouse IgG) at 1ug/ml
Add anti-mouse IgG (Fc specific)-HRP: 1:5000
ELISA analysis specificity of MMAE&MMAF mAb
Coating ADC: 1μg/ml
Add anti-MMAE&MMAF mAb (biotin conjugated): from 1μg/ml, 3-fold diluent, 7 points
Add SA-HRP: 1:10000
Sandwich ELISA assay
Coating anti-MMAE&MMAF mAb: 5μg/ml
Sample: Disitamab vedotin (MMAE ADC) and Belantamab mafodotin (MMAF ADC), from 12.5ng/ml, 2-fold diluent, 7 points
Add anti human Fc HRP: 1:4000