Mouse anti-FOXP3 Recombinant Monoclonal Antibody(R149)
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Mouse anti-FOXP3 Recombinant Monoclonal Antibody(R149)

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品牌: Absin
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来源宿主:
Mouse
实验应用:
WB: 1:1000, IHC-P: 1:500
产品介绍
产品信息
荧光素标记
纯化方式
Protein G
宿主
Mouse
免疫原
N/A
同种型
IgG1
克隆号
R149
克隆类别
Monoclonal Antibody
浓度
2 mg/mL
应用
实验应用
WB: 1:1000, IHC-P: 1:500
背景
翻译后修饰
Unmodified
细胞定位
Nucleus
制备和贮存
存储溶液

PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300

保存方式
Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

参考图片

IHC shows positive staining in paraffin-embedded human stomach. Anti-FOXP3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human Hodgkin's lymphoma. Anti-FOXP3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-FOXP3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

WB result of FOXP3 Mouse mAb Primary antibody: FOXP3 Mouse mAb at 1/1000 dilution Lane 1: 293F whole cell lysate 5 µg Lane 2: 293F transfected with FOXP3 whole cell lysate 5 µg Secondary antibody: abs20039 at 1/10000 dilution Predicted MW: 45kDa Observed MW: 45kDa Exposure time: 10s

IHC shows positive staining in paraffin-embedded human tonsil. Anti-FOXP3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human stomach. Anti-FOXP3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human Hodgkin's lymphoma. Anti-FOXP3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-FOXP3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

WB resuLt of FOXP3 Mouse mAb 
Primary antibody: FOXP3 Mouse mAb at 1/1000 dilution
Lane 1: 293F whole cell lysate 5 µg
Lane 2: 293F transfected with FOXP3 whole cell lysate 5 µg
Secondary antibody: Goat Anti-Mouse IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 45kDa
Observed MW: 45kDa

IHC shows positive staining in paraffin-embedded human tonsil. Anti-FOXP3 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

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货号:
abs172172-1mL
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