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产品介绍
产品介绍
产品信息
荧光素标记
Unconjugated

抗原名称
IgG light chain

来源纯化
Monoclonal antibody is produced by immunizing animals with native total rabbit IgG.

宿主
Mouse

商品描述
Product Usage Information
Recommended Antibody Dilution:1:2000

同种型
Mouse

应用
反应种属
Rabbit

目标/特异性
Specificity/Sensitivity
Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb recognizes the light chain of rabbit IgG, either in its native conformation or in its denatured and reduced form. It does not bind to the denatured and reduced rabbit IgG heavy chain (about 50 kDa) on western blot.
Species Reactivity:
Rabbit

背景
背景
Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb only reacts with the light chain of rabbit IgG and does not bind to the denatured and reduced rabbit IgG heavy chain. When performing immunoprecipitation (IP) followed by western blotting, the denatured rabbit IgG heavy chain of the primary antibody used for IP runs at approximately 50 kDa on the subsequent western blot and can often obscure bands of proteins that have a similar molecular weight. Using Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb as a secondary antibody will eliminate this problem.

翻译后修饰
unmodified

制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
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