首页 Mouse Anti-Rabbit IgG (Light-Chain Specific) (D4W3E) mAb (HRP Conjugate)
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产品信息
来源纯化
Monoclonal antibody is produced by immunizing animals with native total rabbit IgG.
简单描述
This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups.
商品描述
Product Usage Information
Recommended Antibody Dilutions:1:1000-1:3000
应用
目标/特异性
Specificity/Sensitivity
Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb (HRP Conjugate) recognizes the light chain of rabbit IgG, either in its native conformation or in its denatured and reduced form. It does not bind to the denatured and reduced rabbit IgG heavy chain (about 50 kDa) on western blot.
Species Reactivity:
Rabbit
背景
背景
Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb (HRP Conjugate) only reacts with the light chain of rabbit IgG and does not bind to the denatured and reduced rabbit IgG heavy chain. When performing immunoprecipitation (IP) followed by western blotting, the denatured rabbit IgG heavy chain of the primary antibody used for IP runs at approximately 50 kDa on the subsequent western blot and can often obscure bands of proteins that have a similar molecular weight. Using Mouse Anti-rabbit IgG (Light-Chain Specific) (D4W3E) mAb (HRP Conjugate) as a secondary antibody will eliminate this problem.
制备和贮存
保存方式
Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
参考图片
Immunoprecipitation of β-catenin from HeLa cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control (Sepharose® Bead Conjugate) #3423, and lane 3 is β-Catenin (D10A8) XP® Rabbit mAb (Sepharose® Bead Conjugate) #12475. Western blot analysis was performed using β-Catenin (D10A8) XP® Rabbit mAb #8480 as the primary antibody and Mouse Anti-Rabbit IgG (Light-Chain Specific) (D4W3E) mAb (HRP Conjugate) as the secondary antibody.
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