Mouse CSF-1/M-CSF Recombinant Protein

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Mouse CSF-1/M-CSF Recombinant Protein

品牌： CST

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产品介绍

产品信息

来源纯化

Recombinant mouse CSF-1/M-CSF was expressed in E. coli and is supplied in a lyophilized form.

宿主

E. coli

简单描述

MW (kDa)	18.2
Purity	A greater than or equal to 95% purity was determined by SDS-PAGE.
Endotoxin	Endotoxin levels are less than or equal to 1 EU / 1 μg mCSF-1/M-CSF.
Activity	The bioactivity of recombinant mCSF-1/M-CSF was determined in an NFS-60 cell proliferation assay. The ED50 of each lot is less than or equal to 10 ng/ml.

分子量

18.2

研究领域

纤维化,免疫学和肿瘤学,神经科学

应用

反应种属

Mouse

背景

Colony stimulating factor 1 (CSF-1)/macrophage colony stimulating factor (M-CSF) is produced by fibroblasts, endothelial cells, stromal cells, macrophages, osteoblasts, and other cell types (1). CSF-1/M-CSF is required for growth and differentiation of monocytes and macrophages (1,2). CSF-1/M-CSF polarizes macrophages into the M2 phenotype where anti-inflammatory IL-10 is produced, rather than the M1 phenotype where inflammatory cytokines are produced. CSF-1/M-CSF also recruits monocytes and enhances angiogenesis by inducing VEGF production (1,2). CSF-1/M-CSF binds to its receptor CSF1R; downstream signaling involves PI3K/Akt, Erk, and Stats 1, 3, and 5 (1,3). An increase in CSF-1/M-CSF expression may contribute to cancer progression, and high plasma CSF-1/M-CSF levels are associated with rheumatoid arthritis (1,4,5). 1.Hamilton, J.A. (2008) Nat Rev Immunol 8, 533-44. 2.Curry, J.M. et al. (2008) PLoS One 3, e3405. 3.Hamilton, J.A. (1997) J Leukoc Biol 62, 145-55. 4.Rioja, I. et al. (2008) Arthritis Rheum 58, 2257-67. 5.Skrzypski, M. et al. (2008) Clin Cancer Res 14, 4794-9.

研究领域

纤维化,免疫学和肿瘤学,神经科学

翻译后修饰

unmodified

制备和贮存

保存方式

Mouse CSF-1/M-CSF Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.

数据库链接

Entrez-Gene ID

12977

UniProt ID

P07141

参考图片

The purity of Mouse CSF-1/M-CSF Recombinant Protein was determined by SDS-PAGE of 1 µg reduced (+) and non-reduced (-) recombinant mCSF-1/M-CSF and staining with Coomassie Blue. mCSF-1/M-CSF is a homodimer with a predicted total molecular weight (MW) of 36.4 kDa with each subunit equaling 18.2 kDa.

Serial dilutions of Mouse CSF-1/M-CSF Recombinant Protein were added to NFS-60 cells. Cell proliferation was measured and the linear portion of the curve was used to calculate the ED50.

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货号：

33444S

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询价

10ug

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